Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22330%2F25%3A43932231" target="_blank" >RIV/60461373:22330/25:43932231 - isvavai.cz</a>

Výsledek na webu

<a href="https://link.springer.com/article/10.1007/s12223-025-01304-1" target="_blank" >https://link.springer.com/article/10.1007/s12223-025-01304-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-025-01304-1" target="_blank" >10.1007/s12223-025-01304-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay

Popis výsledku v původním jazyce

Nanofibrous materials (NMs), widely used in medical and food industry applications, are highly susceptible to colonisation by microorganisms, including yeasts. Although yeasts can form dense biofilms, methods for studying their metabolic activity remain limited. This study is the first to apply the MTT assay, a standardised method for assessing cell metabolic activity, to evaluate the metabolic activity of yeast biofilm-forming cells on electrospun NMs. First, the biofilm formation of Candida albicans and Saccharomyces cerevisiae on NMs electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined. Then, key parameters of the MTT assay were systematically evaluated: (i) the addition of glucose to the MTT solution, (ii) the presence of menadione in the MTT solution, and (iii) the incubation time with the MTT solution. The addition of glucose was not proven necessary; however, in some cases, it may help distinguish the number of metabolically active cells. Based on this study, we recommend incubation with an MTT solution containing menadione for 2 h. To verify the protocol, colony-forming unit (CFU) enumeration was employed as a reference method. As differences between the results of these two methods were observed, the MTT assay should be complemented by other standardised methods. Nevertheless, the refined protocol offers a solid basis for investigating interactions between NMs and yeasts. © The Author(s) 2025.

Název v anglickém jazyce

Assessing metabolic activity of yeast biofilm-forming cells on nanofibrous materials using MTT assay

Popis výsledku anglicky

Nanofibrous materials (NMs), widely used in medical and food industry applications, are highly susceptible to colonisation by microorganisms, including yeasts. Although yeasts can form dense biofilms, methods for studying their metabolic activity remain limited. This study is the first to apply the MTT assay, a standardised method for assessing cell metabolic activity, to evaluate the metabolic activity of yeast biofilm-forming cells on electrospun NMs. First, the biofilm formation of Candida albicans and Saccharomyces cerevisiae on NMs electrospun from polycaprolactone (PCL), polylactic acid (PLA), and polyamide (PA) was examined. Then, key parameters of the MTT assay were systematically evaluated: (i) the addition of glucose to the MTT solution, (ii) the presence of menadione in the MTT solution, and (iii) the incubation time with the MTT solution. The addition of glucose was not proven necessary; however, in some cases, it may help distinguish the number of metabolically active cells. Based on this study, we recommend incubation with an MTT solution containing menadione for 2 h. To verify the protocol, colony-forming unit (CFU) enumeration was employed as a reference method. As differences between the results of these two methods were observed, the MTT assay should be complemented by other standardised methods. Nevertheless, the refined protocol offers a solid basis for investigating interactions between NMs and yeasts. © The Author(s) 2025.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10612 - Mycology

Projekt

<a href="/cs/project/GA23-05154S" target="_blank" >GA23-05154S: Interakce prokaryotických a eukaryotických buněk s nanovlákny s různou morfologií a strukturou</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

—

Svazek periodika

neuveden

Číslo periodika v rámci svazku

July 2025

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

nestránkováno

Kód UT WoS článku

001537751700001

EID výsledku v databázi Scopus

2-s2.0-105012182970