Characterisation of Ho-DTPA and Y-DTPA complexes by capillary isotachophoresis and TLC with radiometric detection

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F03%3A00007466" target="_blank" >RIV/60461373:22340/03:00007466 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Characterisation of Ho-DTPA and Y-DTPA complexes by capillary isotachophoresis and TLC with radiometric detection

Popis výsledku v původním jazyce

The complexation of holmium and yttrium with DTPA (diethylentriaminepentaacetic acid) was studied as a first step for labelling of monoclonal antibodies for radiopharmaceutical application. Thin-layer chromatography (TLC) with radiometric detection was used to determine the suitable conditions for the preparation of studied complexes. The optimum pH of the reaction mixture, radionuclide ligand ratio, reaction time and stability of selected complex compounds were found. To find out the physico-chemical properties (stability constants) of Ho-DTPA and Y-DTPA complexes the capillary isotachophoresis (cITP) with conductivity detection was applied using the fact that a good linear relationship exists between the stability constants of lanthanide-DTPA complexes and the zone reduction due to tohe blleding effect (liberation of free metal ion). Satisfactory agreement between the stability constant values of Ho-DTPA and Y-DTPA complexes determined by ITP and ones obtained from the JSC Querry Dat

Název v anglickém jazyce

Characterisation of Ho-DTPA and Y-DTPA complexes by capillary isotachophoresis and TLC with radiometric detection

Popis výsledku anglicky

The complexation of holmium and yttrium with DTPA (diethylentriaminepentaacetic acid) was studied as a first step for labelling of monoclonal antibodies for radiopharmaceutical application. Thin-layer chromatography (TLC) with radiometric detection was used to determine the suitable conditions for the preparation of studied complexes. The optimum pH of the reaction mixture, radionuclide ligand ratio, reaction time and stability of selected complex compounds were found. To find out the physico-chemical properties (stability constants) of Ho-DTPA and Y-DTPA complexes the capillary isotachophoresis (cITP) with conductivity detection was applied using the fact that a good linear relationship exists between the stability constants of lanthanide-DTPA complexes and the zone reduction due to tohe blleding effect (liberation of free metal ion). Satisfactory agreement between the stability constant values of Ho-DTPA and Y-DTPA complexes determined by ITP and ones obtained from the JSC Querry Dat

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Physical Chemistry A

ISSN

1089-5639

e-ISSN

—

Svazek periodika

53

Číslo periodika v rámci svazku

53

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

"A63"-"A69"

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—