Speciační analýza selenu v lidském séru

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F05%3A00014719" target="_blank" >RIV/60461373:22340/05:00014719 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Speciation Analysis of Selenium in Human Serum

Popis výsledku v původním jazyce

A post-column isotope dilution analysis has been applied to carry out the quantitative speciation of selenium in human serum by ion exchange chromatography (IEC) coupled to inductively coupled plasma mass spectrometry (ICP-MS). Identification of some selenium-containing proteins was carrying out by affinity chromatography (Hi-Trap Heparin and Hi-Trap Blue-Sepharose columns). Quantification of selenium was performed by post-column isotope dilution analysis by continuous mixing of an enriched 82Se spike solution with the eluent from the column. The 77Se/82Se isotope ratio was monitored and the amount of selenium bound to proteins was evaluated. Three main selenium fractions could be separated and quantified: selenoprotein P, albumin and glutathione peroxidase.

Název v anglickém jazyce

Speciation Analysis of Selenium in Human Serum

Popis výsledku anglicky

A post-column isotope dilution analysis has been applied to carry out the quantitative speciation of selenium in human serum by ion exchange chromatography (IEC) coupled to inductively coupled plasma mass spectrometry (ICP-MS). Identification of some selenium-containing proteins was carrying out by affinity chromatography (Hi-Trap Heparin and Hi-Trap Blue-Sepharose columns). Quantification of selenium was performed by post-column isotope dilution analysis by continuous mixing of an enriched 82Se spike solution with the eluent from the column. The 77Se/82Se isotope ratio was monitored and the amount of selenium bound to proteins was evaluated. Three main selenium fractions could be separated and quantified: selenoprotein P, albumin and glutathione peroxidase.

Druh

A - Audiovizuální tvorba

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

ISBN

—

Místo vydání

Pardubice

Název nakladatele resp. objednatele

Universita Pardubice

Verze

—

Identifikační číslo nosiče

1