Modelování reakčně transportních procesů v mikrokapilárním biosenzoru pro detekci lidských IgG

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F06%3A00016652" target="_blank" >RIV/60461373:22340/06:00016652 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Modeling reaction-transport processes in a microcapillary biosensor for detection of human IgG

Popis výsledku v původním jazyce

Mathematical modeling of electrokinetic transport processes and antigen-antibody interactions in a microfluidic biosensor for heterogeneous immunoassay is described. Two mathematical models of externally controlled electroosmotic transport are constructed - the slip model based on Helmholtz-Smoluchowski approximation and the non-slip model based on the use of Poisson equation. It was found that the use of the slip model is restricted by concentration of the carrier electrolyte and dimensions of the system. The antigen-antibody interaction cannot be described by the slip model. Dynamics of the heterogeneous immunoassay in the proposed biosensor was also studied. Human-IgG-Protein A was used as model system. Parametric studies revealed strong sensitivityof the biosensor to the antibody charge number and to the character of surface heterogeneity in the position of immobilized antigen. This sensitivity can be one of the reasons why the practical applications of heterogeneous protein chips

Název v anglickém jazyce

Modeling reaction-transport processes in a microcapillary biosensor for detection of human IgG

Popis výsledku anglicky

Mathematical modeling of electrokinetic transport processes and antigen-antibody interactions in a microfluidic biosensor for heterogeneous immunoassay is described. Two mathematical models of externally controlled electroosmotic transport are constructed - the slip model based on Helmholtz-Smoluchowski approximation and the non-slip model based on the use of Poisson equation. It was found that the use of the slip model is restricted by concentration of the carrier electrolyte and dimensions of the system. The antigen-antibody interaction cannot be described by the slip model. Dynamics of the heterogeneous immunoassay in the proposed biosensor was also studied. Human-IgG-Protein A was used as model system. Parametric studies revealed strong sensitivityof the biosensor to the antibody charge number and to the character of surface heterogeneity in the position of immobilized antigen. This sensitivity can be one of the reasons why the practical applications of heterogeneous protein chips

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

<a href="/cs/project/GP104%2F03%2FD006" target="_blank" >GP104/03/D006: Studium mikroreaktorů pro bioinženýrské aplikace</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microelectronic Engineering

ISSN

0167-9317

e-ISSN

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Svazek periodika

83

Číslo periodika v rámci svazku

4-9

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

4

Strana od-do

1660-1663

Kód UT WoS článku

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EID výsledku v databázi Scopus

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