Potenciometrický biosenzor s PVC membránou funkcionalizovanou ss oligonukleotidem

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F07%3A00019293" target="_blank" >RIV/60461373:22340/07:00019293 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22310/07:00019293 RIV/60461373:22320/07:00019293

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor

Popis výsledku v původním jazyce

A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)15, modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)15 on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA+Cl-). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)15 modified membranes were sensitive toward complementary oligo(dT)15 in the concentration range 2-80nM at pH 7. An explanation for the detection mechanism is proposed.

Název v anglickém jazyce

Functionalization of PVC membrane with ss oligonucleotides for a potentiometric biosensor

Popis výsledku anglicky

A novel application of a single stranded (ss) oligonucleotide as an active component of polymeric membrane in an ion-selective electrode (ISE) is described. The original oligonucleotides, oligo(dA)15, modified by cholesterol, triphenylmethyl and hexadecyl derivatives, were immobilized into poly(vinyl chloride) (PVC) membrane using extraction protocol. In parallel, the adsorption protocol was used to immobilize unmodified oligo(dA)15 on the PVC membrane based on tridodecylmethyammonium chloride (TDDMA+Cl-). Immobilization of ss oligonucleotide probe through spacer was more effective for the potentiometric detection of the hybridization between complementary oligonucleotides. It was found that cholesterol-oligo(dA)15 modified membranes were sensitive toward complementary oligo(dT)15 in the concentration range 2-80nM at pH 7. An explanation for the detection mechanism is proposed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2007

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biosensors and Bioelectronics

ISSN

0956-5663

e-ISSN

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Svazek periodika

22

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

6

Strana od-do

2712-2717

Kód UT WoS článku

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EID výsledku v databázi Scopus

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