On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F09%3A00022071" target="_blank" >RIV/60461373:22340/09:00022071 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

Popis výsledku v původním jazyce

Radio-frequency impedance based biomass measurements represent a relatively accurate and reliable method for on-line quantification of viable cellular biomass. For the living biomass concentration determination, a linear correlation between biomass concentration and capacitance is generally assumed. However, this assumption is not always valid as e.g. the presence of intracellular lipid inclusions may violate the assumption of linear correlation. Therefore, the main objective of this study was to examine the potential of the sensor for on-line in-situ monitoring of intracellular biopolymer content (PHA) in bacterial cells. It was experimentally confirmed that the loss of linearity between dry cell weight and capacitance takes place also in the case ofthe Pseudomonas putida culture. The resulting nonlinear behaviour can be used for the design of a novel biopolymer content estimator based on the capacitance and offgas composition measurements.

Název v anglickém jazyce

On-line Monitoring of PHA Cellular Content in Pseudomonas putida Cultures Using Radio-frequency Impedance

Popis výsledku anglicky

Radio-frequency impedance based biomass measurements represent a relatively accurate and reliable method for on-line quantification of viable cellular biomass. For the living biomass concentration determination, a linear correlation between biomass concentration and capacitance is generally assumed. However, this assumption is not always valid as e.g. the presence of intracellular lipid inclusions may violate the assumption of linear correlation. Therefore, the main objective of this study was to examine the potential of the sensor for on-line in-situ monitoring of intracellular biopolymer content (PHA) in bacterial cells. It was experimentally confirmed that the loss of linearity between dry cell weight and capacitance takes place also in the case ofthe Pseudomonas putida culture. The resulting nonlinear behaviour can be used for the design of a novel biopolymer content estimator based on the capacitance and offgas composition measurements.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

JD - Využití počítačů, robotika a její aplikace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

New Biotechnology

ISSN

1871-6784

e-ISSN

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Svazek periodika

25

Číslo periodika v rámci svazku

S1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

2

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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