Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F10%3A00023418" target="_blank" >RIV/60461373:22340/10:00023418 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida

Popis výsledku v původním jazyce

Fucoidan from the sporophyll (Miyeokgui) of cultured Korean brown seaweeds Undaria pinnatifida (Miyeok) is interesting due to its various biological activities. This polysaccharide was isolated from raw material by dilute acid extraction, ballast alginates were removed by CaCl2 precipitation, and crude extract was purified by chromatography on DEAE-cellulose. Structure and composition of the fucoidan was characterised by various methods (organic elemental analysis, HPLC analysis of neutral sugars, FTIR,FT-Raman, H-1 and C-13 NMR); molecular size and charge density were estimated by GPC and CITP. The polysaccharide was eluted by GPC as a single peak of approximate molecular weight of 1246 kDa. Miyeokgui fucoidan showed lower electrophoretic mobility (RSH similar to 0.127) than carrageenans (0.096-0.100) and chondroitin sulphate A (0.103) but higher than alginate (0.170). Isolated polysaccharide contained alpha-fucose (50.9 mol%) and beta-galactose (44.6 mol%) as main neutral sugar unit

Název v anglickém jazyce

Structure and antitumour activity of fucoidan isolated from sporophyll of Korean brown seaweed Undaria pinnatifida

Popis výsledku anglicky

Fucoidan from the sporophyll (Miyeokgui) of cultured Korean brown seaweeds Undaria pinnatifida (Miyeok) is interesting due to its various biological activities. This polysaccharide was isolated from raw material by dilute acid extraction, ballast alginates were removed by CaCl2 precipitation, and crude extract was purified by chromatography on DEAE-cellulose. Structure and composition of the fucoidan was characterised by various methods (organic elemental analysis, HPLC analysis of neutral sugars, FTIR,FT-Raman, H-1 and C-13 NMR); molecular size and charge density were estimated by GPC and CITP. The polysaccharide was eluted by GPC as a single peak of approximate molecular weight of 1246 kDa. Miyeokgui fucoidan showed lower electrophoretic mobility (RSH similar to 0.127) than carrageenans (0.096-0.100) and chondroitin sulphate A (0.103) but higher than alginate (0.170). Isolated polysaccharide contained alpha-fucose (50.9 mol%) and beta-galactose (44.6 mol%) as main neutral sugar unit

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Carbohydrate Polymers

ISSN

0144-8617

e-ISSN

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Svazek periodika

81

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

IE - Irsko

Počet stran výsledku

8

Strana od-do

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Kód UT WoS článku

000277953500007

EID výsledku v databázi Scopus

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