The minimizing of fluorescence background in Raman optical activity and Raman spectra of human blood plasma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43899094" target="_blank" >RIV/60461373:22340/15:43899094 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/15:10294734 RIV/00064165:_____/15:10294734

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00216-014-8358-7" target="_blank" >http://dx.doi.org/10.1007/s00216-014-8358-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-014-8358-7" target="_blank" >10.1007/s00216-014-8358-7</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The minimizing of fluorescence background in Raman optical activity and Raman spectra of human blood plasma

Popis výsledku v původním jazyce

Raman optical activity (ROA) is inherently sensitive to the secondary structure of biomolecules, which makes it a method of interest for finding new approaches to clinical applications based on blood plasma analysis, for instance the diagnostics of several protein-misfolding diseases. Unfortunately, real blood plasma exhibits strong background fluorescence when excited at 532 nm; hence, measuring the ROA spectra appears to be impossible. Therefore, we established a suitable method using a combination of kinetic quenchers, filtering, photobleaching, and a mathematical correction of residual fluorescence. Our method reduced the background fluorescence approximately by 90 %, which allowed speedup for each measurement by an average of 50 %. In addition, the signal-to-noise ratio was significantly increased, while the baseline distortion remained low. We assume that our method is suitable for the investigation of human blood plasma by ROA and may lead to the development of a new tool for clinical diagnostics.

Název v anglickém jazyce

The minimizing of fluorescence background in Raman optical activity and Raman spectra of human blood plasma

Popis výsledku anglicky

Raman optical activity (ROA) is inherently sensitive to the secondary structure of biomolecules, which makes it a method of interest for finding new approaches to clinical applications based on blood plasma analysis, for instance the diagnostics of several protein-misfolding diseases. Unfortunately, real blood plasma exhibits strong background fluorescence when excited at 532 nm; hence, measuring the ROA spectra appears to be impossible. Therefore, we established a suitable method using a combination of kinetic quenchers, filtering, photobleaching, and a mathematical correction of residual fluorescence. Our method reduced the background fluorescence approximately by 90 %, which allowed speedup for each measurement by an average of 50 %. In addition, the signal-to-noise ratio was significantly increased, while the baseline distortion remained low. We assume that our method is suitable for the investigation of human blood plasma by ROA and may lead to the development of a new tool for clinical diagnostics.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT13259" target="_blank" >NT13259: Význam vyšetřování plasmatických hladin angiogenetických a imunosupresivních faktorů TGF beta1, VEGF a imunitních buněk uplatňujících se v protinádorové odpovědi (NK, CD8) u pacientů s karcinomem tlustého střeva.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Svazek periodika

407

Číslo periodika v rámci svazku

5

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

8

Strana od-do

1335-1342

Kód UT WoS článku

000349336400008

EID výsledku v databázi Scopus

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