The potential of chiroptical and vibrational spectroscopy of blood plasma for the discrimination between colon cancer patients and the control group

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43899271" target="_blank" >RIV/60461373:22340/15:43899271 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/15:10294888 RIV/00064165:_____/15:10294888

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c4an01880j" target="_blank" >http://dx.doi.org/10.1039/c4an01880j</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c4an01880j" target="_blank" >10.1039/c4an01880j</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The potential of chiroptical and vibrational spectroscopy of blood plasma for the discrimination between colon cancer patients and the control group

Popis výsledku v původním jazyce

Colorectal cancer is one of the most abundant causes of cancer deaths in the world. At an early stage, the established clinical procedures have low reliability and sensitivity. Therefore, we tested a novel approach based on chiroptical methods such as electronic circular dichroism (ECD) and Raman optical activity (ROA). These methods are suitable for detecting slight changes in the 3D structure of chiral biomolecules, some of which may be caused by pathological processes occurring during cancer growth. Fifty-five blood plasma samples were analyzed using the combination of ECD and ROA supplemented by conventional Raman and FT-IR spectroscopy. All obtained spectra were evaluated together by linear discriminant analysis. The accuracy of sample discrimination reached 100% and the subsequent leave-one-out cross-validation resulted in 93% sensitivity and 81% specificity. The achieved results indicate that chiroptical methods supplemented by Raman and FT-IR spectroscopy might be new supporting and minimally invasive tools in the clinical diagnosis of colon cancer.

Název v anglickém jazyce

The potential of chiroptical and vibrational spectroscopy of blood plasma for the discrimination between colon cancer patients and the control group

Popis výsledku anglicky

Colorectal cancer is one of the most abundant causes of cancer deaths in the world. At an early stage, the established clinical procedures have low reliability and sensitivity. Therefore, we tested a novel approach based on chiroptical methods such as electronic circular dichroism (ECD) and Raman optical activity (ROA). These methods are suitable for detecting slight changes in the 3D structure of chiral biomolecules, some of which may be caused by pathological processes occurring during cancer growth. Fifty-five blood plasma samples were analyzed using the combination of ECD and ROA supplemented by conventional Raman and FT-IR spectroscopy. All obtained spectra were evaluated together by linear discriminant analysis. The accuracy of sample discrimination reached 100% and the subsequent leave-one-out cross-validation resulted in 93% sensitivity and 81% specificity. The achieved results indicate that chiroptical methods supplemented by Raman and FT-IR spectroscopy might be new supporting and minimally invasive tools in the clinical diagnosis of colon cancer.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NT13259" target="_blank" >NT13259: Význam vyšetřování plasmatických hladin angiogenetických a imunosupresivních faktorů TGF beta1, VEGF a imunitních buněk uplatňujících se v protinádorové odpovědi (NK, CD8) u pacientů s karcinomem tlustého střeva.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analyst

ISSN

0003-2654

e-ISSN

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Svazek periodika

140

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

2287-2293

Kód UT WoS článku

000351405900028

EID výsledku v databázi Scopus

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