Inactivation of human pathogenic dermatophytes by non-thermal plasma

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F15%3A43899499" target="_blank" >RIV/60461373:22340/15:43899499 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388971:_____/15:00456143 RIV/00216208:11310/15:10314405 RIV/00216208:11110/15:10314405

Výsledek na webu

<a href="http://www.sciencedirect.com/science/article/pii/S0167701215300737" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0167701215300737</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.mimet.2015.09.017" target="_blank" >10.1016/j.mimet.2015.09.017</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Inactivation of human pathogenic dermatophytes by non-thermal plasma

Popis výsledku v původním jazyce

Non-thermal plasma (NTP) was tested as an in vitro deactivation method on four human pathogenic dermatophytes belonging to all ecological groups including anthropophilic Trichophyton rubrum and Trichophyton interdigitale, zoophilic Arthroderma benhamiae, and geophilic Microsporum gypseum. The identification of all strains was confirmed by sequencing of ITS rDNA region (internal transcribed spacer region of ribosomal DNA). Dermatophyte sporeswere suspended inwater or inoculated on agar plates and exposed to NTP generated by a positive or negative corona discharge, or cometary discharge. After 15 min of exposure to NTP a significant decrease in the number of surviving spores inwater suspensionswas observed in all species. Complete spore inactivation and thus decontamination was observed in anthropophilic species after 25 min of exposure. Similarly, a significant decrease in the number of surviving sporeswas observed after 10-15 min of exposure to NTP on the surface of agar plateswith full inhibition after 25 min in all tested species except ofM. gypseum. Although the sensitivity of dermatophytes to the action of NTP appears to be lower than that of bacteria and yeast, our results suggest that NTP has the potential to be used as an alternative treatment strategy for dermatophytosis and could be useful for surface decontamination in clinical practice.

Název v anglickém jazyce

Inactivation of human pathogenic dermatophytes by non-thermal plasma

Popis výsledku anglicky

Non-thermal plasma (NTP) was tested as an in vitro deactivation method on four human pathogenic dermatophytes belonging to all ecological groups including anthropophilic Trichophyton rubrum and Trichophyton interdigitale, zoophilic Arthroderma benhamiae, and geophilic Microsporum gypseum. The identification of all strains was confirmed by sequencing of ITS rDNA region (internal transcribed spacer region of ribosomal DNA). Dermatophyte sporeswere suspended inwater or inoculated on agar plates and exposed to NTP generated by a positive or negative corona discharge, or cometary discharge. After 15 min of exposure to NTP a significant decrease in the number of surviving spores inwater suspensionswas observed in all species. Complete spore inactivation and thus decontamination was observed in anthropophilic species after 25 min of exposure. Similarly, a significant decrease in the number of surviving sporeswas observed after 10-15 min of exposure to NTP on the surface of agar plateswith full inhibition after 25 min in all tested species except ofM. gypseum. Although the sensitivity of dermatophytes to the action of NTP appears to be lower than that of bacteria and yeast, our results suggest that NTP has the potential to be used as an alternative treatment strategy for dermatophytosis and could be useful for surface decontamination in clinical practice.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BL - Fyzika plasmatu a výboje v plynech

OECD FORD obor

—

Projekt

<a href="/cs/project/ED1.1.00%2F02.0109" target="_blank" >ED1.1.00/02.0109: Biotechnologické a biomedicínské centrum Akademie věd a Univerzity Karlovy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Microbiological Methods

ISSN

0167-7012

e-ISSN

—

Svazek periodika

119

Číslo periodika v rámci svazku

December

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

53-58

Kód UT WoS článku

000366442400009

EID výsledku v databázi Scopus

—