Formation of 3D multicellular spheroids in liquid marble

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43902161" target="_blank" >RIV/60461373:22340/16:43902161 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Formation of 3D multicellular spheroids in liquid marble

Popis výsledku v původním jazyce

We successfully proved the creation of spheroids by human colorectal adenocarcinoma cell line (HT-29) by usage of the liquid marbles, liquid droplets encapsulated by hydrophobic powder. During the cultivation cells spontaneously formed the spherical agglomerates (spheroids) without the scaffold. The study focused on the influence of different parameters (volume, cell density, day) on final harvest. To evaluate the harvest we counted the number of created spheroids and the total number of cells. Better simulation of living body is essential to increase the efficiency of drug development. In vivo conditions are significantly different comparing to in vitro conditions. Today used cell monolayers (2D structures of cells) are not accurate enough to simulate living organism, but growing cells as 3D structure have more potential. Drug absorption, distribution, metabolism, excretion and toxicity could be tested on these 3D multicellular spheroids, decrease the use of animal models and accelerate the drug development.

Název v anglickém jazyce

Formation of 3D multicellular spheroids in liquid marble

Popis výsledku anglicky

We successfully proved the creation of spheroids by human colorectal adenocarcinoma cell line (HT-29) by usage of the liquid marbles, liquid droplets encapsulated by hydrophobic powder. During the cultivation cells spontaneously formed the spherical agglomerates (spheroids) without the scaffold. The study focused on the influence of different parameters (volume, cell density, day) on final harvest. To evaluate the harvest we counted the number of created spheroids and the total number of cells. Better simulation of living body is essential to increase the efficiency of drug development. In vivo conditions are significantly different comparing to in vitro conditions. Today used cell monolayers (2D structures of cells) are not accurate enough to simulate living organism, but growing cells as 3D structure have more potential. Drug absorption, distribution, metabolism, excretion and toxicity could be tested on these 3D multicellular spheroids, decrease the use of animal models and accelerate the drug development.

Druh

D - Stať ve sborníku

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

<a href="/cs/project/GA13-37055S" target="_blank" >GA13-37055S: Dalkové ovládání adheze a reakčně-difuzních procesů ve strukturovaných mikročásticích</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceedings 43rd International Conference of the Slovak Society of Chemical Engineering

ISBN

978-80-89597-35-2

ISSN

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e-ISSN

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Počet stran výsledku

8

Strana od-do

283-290

Název nakladatele

Slovak Society of Chemical Engineering

Místo vydání

Bratislava

Místo konání akce

Tatranské Matliare

Datum konání akce

23. 5. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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