Discrete Turing Patterns in Coupled Reaction Cells in a Cyclic Array

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43903030" target="_blank" >RIV/60461373:22340/16:43903030 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s11144-016-1004-y" target="_blank" >http://dx.doi.org/10.1007/s11144-016-1004-y</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11144-016-1004-y" target="_blank" >10.1007/s11144-016-1004-y</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Discrete Turing Patterns in Coupled Reaction Cells in a Cyclic Array

Popis výsledku v původním jazyce

Our work is focused on experimental observation of nonuniform stationary states (discrete Turing patterns) in a cyclic coupled cell array with glycolytic oscillatory reaction. Mutual mass exchange between two stirred reaction cells is realized by using a peristaltic pump delivering reciprocally the reaction mixture in both directions. The cells are fed by solutions of yeast extract and D-glucose. The cyclic system is formed by the two cells and the tubing, connecting the cells, acting as additional two reactors because of a non-negligible transport time. Experiments are conducted under 45oC and 50oC to thermally activate positive and negative feedback loops of the enzyme phosphofructokinase and thus achieve conditions appropriate for occurrence of the patterns. Dynamical state in one cell of the ring is measured using UV-Vis spectrophotometer reading absorbance levels of adenosine pool together with NAD+ (260nm) and absorbance level of NADH (340 nm). We report transitions between uniform stationary states, nonuniform stationary states (discrete Turing patterns) and uniform oscillations. We also performed analysis of stability and bifurcations of stationary states in a model of four coupled cells in a cyclic array using a core kinetic model of glycolysis. By examining the space of relevant parameters, we found conditions for occurrence of discrete Turing patterns consistent with our experiments. Solution diagrams are used to display the stationary concentration of ADP as a function of the inhibition rate coefficient and to explain the mechanism by which the discrete Turing patterns occur.

Název v anglickém jazyce

Discrete Turing Patterns in Coupled Reaction Cells in a Cyclic Array

Popis výsledku anglicky

Our work is focused on experimental observation of nonuniform stationary states (discrete Turing patterns) in a cyclic coupled cell array with glycolytic oscillatory reaction. Mutual mass exchange between two stirred reaction cells is realized by using a peristaltic pump delivering reciprocally the reaction mixture in both directions. The cells are fed by solutions of yeast extract and D-glucose. The cyclic system is formed by the two cells and the tubing, connecting the cells, acting as additional two reactors because of a non-negligible transport time. Experiments are conducted under 45oC and 50oC to thermally activate positive and negative feedback loops of the enzyme phosphofructokinase and thus achieve conditions appropriate for occurrence of the patterns. Dynamical state in one cell of the ring is measured using UV-Vis spectrophotometer reading absorbance levels of adenosine pool together with NAD+ (260nm) and absorbance level of NADH (340 nm). We report transitions between uniform stationary states, nonuniform stationary states (discrete Turing patterns) and uniform oscillations. We also performed analysis of stability and bifurcations of stationary states in a model of four coupled cells in a cyclic array using a core kinetic model of glycolysis. By examining the space of relevant parameters, we found conditions for occurrence of discrete Turing patterns consistent with our experiments. Solution diagrams are used to display the stationary concentration of ADP as a function of the inhibition rate coefficient and to explain the mechanism by which the discrete Turing patterns occur.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CI - Průmyslová chemie a chemické inženýrství

OECD FORD obor

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Projekt

<a href="/cs/project/GA15-17367S" target="_blank" >GA15-17367S: Dynamika složitých reakčních sítí v enzymových reaktorech a fotobioreaktorech</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Reaction Kinetics Mechanisms and Catalysis

ISSN

1878-5190

e-ISSN

—

Svazek periodika

118

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

16

Strana od-do

99-114

Kód UT WoS článku

000376107700010

EID výsledku v databázi Scopus

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