Molecular-spectroscopic analysis of blood plasma of patients with pancreatic cancer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F16%3A43903273" target="_blank" >RIV/60461373:22340/16:43903273 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Molecular-spectroscopic analysis of blood plasma of patients with pancreatic cancer

Popis výsledku v původním jazyce

Pancreatic cancer (PC) is one of the most aggressive cancer types; and thus, it represents the most common cause of cancer death. The absence of highly specific biomarkers and the low reliability of current diagnostic procedures do not facilitate a proper early-stage diagnosis. Moreover, the disease is often misdiagnosed, because some of the early-stage symptoms appear similar to those of type II diabetes mellitus (DM2). With the onset of cancer, the changes in content and conformation of several biomolecules, including peptides and proteins, may be observed in blood plasma. Hence, we performed the spectroscopic analysis of blood plasma of patients with PC, DM2 and healthy subjects using chiroptical methods. Raman optical activity (ROA) and electronic circular dichroism (ECD), both of which are inherently sensitive to 3D structure of chiral molecules, were supplemented by non-polarized vibrational techniques, specifically Raman and infrared spectroscopy. We were able to distinguish not only healthy from diseased subjects with high level of accuracy, but we have also identified the variations of the spectral response between PC and DM2. According to the achieved results, the combination of chiroptical and vibrational spectroscopies might have a promising potential for the identification of subjects in the early-stage of PC.

Název v anglickém jazyce

Molecular-spectroscopic analysis of blood plasma of patients with pancreatic cancer

Popis výsledku anglicky

Pancreatic cancer (PC) is one of the most aggressive cancer types; and thus, it represents the most common cause of cancer death. The absence of highly specific biomarkers and the low reliability of current diagnostic procedures do not facilitate a proper early-stage diagnosis. Moreover, the disease is often misdiagnosed, because some of the early-stage symptoms appear similar to those of type II diabetes mellitus (DM2). With the onset of cancer, the changes in content and conformation of several biomolecules, including peptides and proteins, may be observed in blood plasma. Hence, we performed the spectroscopic analysis of blood plasma of patients with PC, DM2 and healthy subjects using chiroptical methods. Raman optical activity (ROA) and electronic circular dichroism (ECD), both of which are inherently sensitive to 3D structure of chiral molecules, were supplemented by non-polarized vibrational techniques, specifically Raman and infrared spectroscopy. We were able to distinguish not only healthy from diseased subjects with high level of accuracy, but we have also identified the variations of the spectral response between PC and DM2. According to the achieved results, the combination of chiroptical and vibrational spectroscopies might have a promising potential for the identification of subjects in the early-stage of PC.

Druh

O - Ostatní výsledky

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/NV16-31028A" target="_blank" >NV16-31028A: Účinnost nových spektroskopických biomarkerů pro detekci časného stádia karcinomu pankreatu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů