Electrochemical Detection of Sialic Acid Using Phenylboronic Acid-Modified Poly (Diaminobenzoic Acid) Electrodes

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F18%3A43915442" target="_blank" >RIV/60461373:22340/18:43915442 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388963:_____/18:00489957 RIV/00216208:11110/18:10376682

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201700634" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201700634</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201700634" target="_blank" >10.1002/elan.201700634</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Electrochemical Detection of Sialic Acid Using Phenylboronic Acid-Modified Poly (Diaminobenzoic Acid) Electrodes

Popis výsledku v původním jazyce

This study is focused on the electrochemical detection of sialic acid (SA) using electrochemical impedance spectroscopy (EIS) and potentiometric approaches with the polymeric film derived from 3,4-diaminobenzoic acid (3,4-DABA) and modified with 3-aminophenylboronic acid (3-APBA). The polymeric film derived from 3,4-DABA was prepared using cyclic voltammetry at 0.5 M H2SO4 on a platinum disc electrode from 0 to 1.20 V vs. Ag/AgCl and characterized by FTIR-Raman spectroscopy. It has been found that the polymerization process goes through the amino group and a free carboxyl group serves for the covalent binding of 3-APBA onto the polymeric backbone. Both the electrochemical approaches independently confirmed the recognition of SA by APBA-modified PDABA electrodes. In contrast to known electrochemical sensors in the literature, the APBA-modified PADABA sensor for SA gave a response in the range from 0.025 to 0.998 mM at neutral pH using the EIS method. The decrease in the EIS signal of ca 12 % was observed after two weeks of intensive use. Importantly, the selective APBA-modified polymeric electrode enabled the use of SA determination in artificial urine samples in a concentration range that is useful for diagnostic purposes, specifically 12–102 μg mL−1.

Název v anglickém jazyce

Electrochemical Detection of Sialic Acid Using Phenylboronic Acid-Modified Poly (Diaminobenzoic Acid) Electrodes

Popis výsledku anglicky

This study is focused on the electrochemical detection of sialic acid (SA) using electrochemical impedance spectroscopy (EIS) and potentiometric approaches with the polymeric film derived from 3,4-diaminobenzoic acid (3,4-DABA) and modified with 3-aminophenylboronic acid (3-APBA). The polymeric film derived from 3,4-DABA was prepared using cyclic voltammetry at 0.5 M H2SO4 on a platinum disc electrode from 0 to 1.20 V vs. Ag/AgCl and characterized by FTIR-Raman spectroscopy. It has been found that the polymerization process goes through the amino group and a free carboxyl group serves for the covalent binding of 3-APBA onto the polymeric backbone. Both the electrochemical approaches independently confirmed the recognition of SA by APBA-modified PDABA electrodes. In contrast to known electrochemical sensors in the literature, the APBA-modified PADABA sensor for SA gave a response in the range from 0.025 to 0.998 mM at neutral pH using the EIS method. The decrease in the EIS signal of ca 12 % was observed after two weeks of intensive use. Importantly, the selective APBA-modified polymeric electrode enabled the use of SA determination in artificial urine samples in a concentration range that is useful for diagnostic purposes, specifically 12–102 μg mL−1.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Svazek periodika

30

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

672-680

Kód UT WoS článku

000429708900013

EID výsledku v databázi Scopus

2-s2.0-85037999868