Preparation of thermoresponsive poly(N-isopropylacrylamide) surfaces for non-enzymatic cell harvesting

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F19%3A43918549" target="_blank" >RIV/60461373:22340/19:43918549 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22350/19:43918549

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of thermoresponsive poly(N-isopropylacrylamide) surfaces for non-enzymatic cell harvesting

Popis výsledku v původním jazyce

The main goal of regenerative medicine is to treat or replace organs, their parts or cells that have been damaged by age, trauma or pathological changes. For such purpose scientists work on the development of modern methods utilizing stem cells. These cells have a unique ability to differentiate in the body into any conventional cell, helping to regenerate damaged tissues. Stem cells originate from two main sources: adult body tissues and embryos. Adult stem cells exist throughout the body from the time an embryo develops. In the developed human body, stem cells can be mainly found in the bone marrow, adipose tissue, and blood. The use of stem cells for clinical applications requires high quality and quantity of cells. Traditional methods for maintenance and expansion of cells rely on culturing techniques using plastic culture plates. The problem arises in the separation of the grown cells from the substrate surface. Conventionally, cells are recovered by mechanical cell scraping or enzymatically induced cell harvesting. Both methods can be very harsh and may lead to cells with impaired cell functionality, which is a major obstacle for approval of cell therapy. The utilization of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) surfaces for cell harvesting can be an excellent alternative to traditional methods, as the cell detachment is mediated exclusively by temperature. The study aims the preparation of the pNIPAAm modified polydimethylsiloxane (PDMS) substrates, characterization of their thermoresponsive behavior and temperature-triggered cell harvesting. Thermoresponsive surfaces of different amount of grafted pNIPAAm were prepared by atom transfer radical polymerization varying the reaction conditions (oxygen plasma PDMS initiation, polymerization time and monomer concentration). Successful NIPAAm grafting was confirmed by the FTIR and XPS analysis. Thermoresponsive wettability changes of pNIPAAm brush surfaces were evaluated by static contact angle measurements. The influence of the amount of the grafted pNIPAAM on mesenchymal stem cell adhesion and detachment is carefully examined.

Název v anglickém jazyce

Preparation of thermoresponsive poly(N-isopropylacrylamide) surfaces for non-enzymatic cell harvesting

Popis výsledku anglicky

The main goal of regenerative medicine is to treat or replace organs, their parts or cells that have been damaged by age, trauma or pathological changes. For such purpose scientists work on the development of modern methods utilizing stem cells. These cells have a unique ability to differentiate in the body into any conventional cell, helping to regenerate damaged tissues. Stem cells originate from two main sources: adult body tissues and embryos. Adult stem cells exist throughout the body from the time an embryo develops. In the developed human body, stem cells can be mainly found in the bone marrow, adipose tissue, and blood. The use of stem cells for clinical applications requires high quality and quantity of cells. Traditional methods for maintenance and expansion of cells rely on culturing techniques using plastic culture plates. The problem arises in the separation of the grown cells from the substrate surface. Conventionally, cells are recovered by mechanical cell scraping or enzymatically induced cell harvesting. Both methods can be very harsh and may lead to cells with impaired cell functionality, which is a major obstacle for approval of cell therapy. The utilization of thermoresponsive poly(N-isopropylacrylamide) (pNIPAAm) surfaces for cell harvesting can be an excellent alternative to traditional methods, as the cell detachment is mediated exclusively by temperature. The study aims the preparation of the pNIPAAm modified polydimethylsiloxane (PDMS) substrates, characterization of their thermoresponsive behavior and temperature-triggered cell harvesting. Thermoresponsive surfaces of different amount of grafted pNIPAAm were prepared by atom transfer radical polymerization varying the reaction conditions (oxygen plasma PDMS initiation, polymerization time and monomer concentration). Successful NIPAAm grafting was confirmed by the FTIR and XPS analysis. Thermoresponsive wettability changes of pNIPAAm brush surfaces were evaluated by static contact angle measurements. The influence of the amount of the grafted pNIPAAM on mesenchymal stem cell adhesion and detachment is carefully examined.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

20402 - Chemical process engineering

Projekt

<a href="/cs/project/EG17_107%2F0012489" target="_blank" >EG17_107/0012489: NewSurf: Nová metoda povrchové úpravy pro neenzymatické sklízení buněk určených k farmakologickému nebo lékařskému použití</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů