Solvatochromic Shift Enables Intracellular Fate Determination of Curcumin Nanocrystals

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F22%3A43924675" target="_blank" >RIV/60461373:22340/22:43924675 - isvavai.cz</a>

Výsledek na webu

<a href="https://doi.org/10.1002/ppsc.202200115" target="_blank" >https://doi.org/10.1002/ppsc.202200115</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/ppsc.202200115" target="_blank" >10.1002/ppsc.202200115</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Solvatochromic Shift Enables Intracellular Fate Determination of Curcumin Nanocrystals

Popis výsledku v původním jazyce

Drug nanocrystals represent an effective way of delivering poorly water-soluble substances to target tissues and cells. Due to the large specific surface area, nanocrystals promote dissolution; therefore, the drug usually co-exists in both crystalline and dissolved forms. Commonly used analytical methods do not make it possible to easily distinguish between these forms within live cells. The present study reports a novel fluorescence microscopy method that makes it possible to distinguish intracellular forms of curcumin based on differences in fluorescence emission spectra. Due to solvatochromism, crystalline curcumin can be distinguished from curcumin dissolved in the lipidic components of cells. The methodology utilization is demonstrated by investigating the effect of surface stabilization of curcumin nanocrystals on cell uptake by different cell lines: macrophages and epithelial cells. The understanding of factors that control drug nanocrystal phagocytosis makes it possible to better interpret the observed anticancer effects of curcumin nanocrystals and design efficient drug delivery systems. © 2022 Wiley-VCH GmbH.

Název v anglickém jazyce

Solvatochromic Shift Enables Intracellular Fate Determination of Curcumin Nanocrystals

Popis výsledku anglicky

Drug nanocrystals represent an effective way of delivering poorly water-soluble substances to target tissues and cells. Due to the large specific surface area, nanocrystals promote dissolution; therefore, the drug usually co-exists in both crystalline and dissolved forms. Commonly used analytical methods do not make it possible to easily distinguish between these forms within live cells. The present study reports a novel fluorescence microscopy method that makes it possible to distinguish intracellular forms of curcumin based on differences in fluorescence emission spectra. Due to solvatochromism, crystalline curcumin can be distinguished from curcumin dissolved in the lipidic components of cells. The methodology utilization is demonstrated by investigating the effect of surface stabilization of curcumin nanocrystals on cell uptake by different cell lines: macrophages and epithelial cells. The understanding of factors that control drug nanocrystal phagocytosis makes it possible to better interpret the observed anticancer effects of curcumin nanocrystals and design efficient drug delivery systems. © 2022 Wiley-VCH GmbH.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

21001 - Nano-materials (production and properties)

Projekt

<a href="/cs/project/GX19-26127X" target="_blank" >GX19-26127X: Robotický nano-lékárník: Výrobní procesy budoucnosti pro personalisovaná terapeutika</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Particle Particle Systéms Characterization

ISSN

0934-0866

e-ISSN

1521-4117

Svazek periodika

39

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

nestrankovano

Kód UT WoS článku

000865838500001

EID výsledku v databázi Scopus

2-s2.0-85137553265