Comparative Study on Handheld, Modular, and Laboratory Raman Instruments for the Analysis of Colon Tissues and Colorectal Polyps

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F24%3A43927927" target="_blank" >RIV/60461373:22340/24:43927927 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/24:10478247 RIV/00064165:_____/24:10478247

Výsledek na webu

<a href="https://doi.org/10.3390/app14020791" target="_blank" >https://doi.org/10.3390/app14020791</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/app14020791" target="_blank" >10.3390/app14020791</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparative Study on Handheld, Modular, and Laboratory Raman Instruments for the Analysis of Colon Tissues and Colorectal Polyps

Popis výsledku v původním jazyce

Portable Raman spectrometers may offer advantages for clinical medical diagnostics overlaboratory instruments by allowing for quick measurements in the field and provision of data suitablefor screening analyses. This work evaluates the potential of using available handheld, modular,and laboratory Raman spectrometers for screening normal colon tissues and benign and malignantcolon polyps. The Raman spectra of tissue samples and reference biological macromolecules weremeasured with these instruments and analyzed using curve fitting and multivariate statistics. Thespectra of calf thymus DNA measured with portable devices showed suitable signal-to-noise levelsand half-widths of the prominent bands. Band positions, resolution, and relative intensities inthe Raman spectra of colon tissues and reference compounds varied for the instruments, and thelaboratory device demonstrated the best spectral feature. The principal component analysis (PCA)of the spectra obtained with all Raman devices showed well discrimination of normal colon tissue,adenomatous polyp, and adenocarcinoma. Dendrograms of similarity obtained using hierarchycluster analysis (HCA) for the Raman spectra of all three devices also showed good separation ofthese samples. The soft independent modeling of class analogy (SIMCA) and support vector machine(SVM) models efficiently classified normal colon tissues and benign/malignant colorectal polypsbased on the Raman data from all three devices. Despite its less pronounced spectral characteristics,the handheld Raman spectrometer can be used in early diagnosis of colorectal carcinoma, comparableto the modular and laboratory instruments.

Název v anglickém jazyce

Comparative Study on Handheld, Modular, and Laboratory Raman Instruments for the Analysis of Colon Tissues and Colorectal Polyps

Popis výsledku anglicky

Portable Raman spectrometers may offer advantages for clinical medical diagnostics overlaboratory instruments by allowing for quick measurements in the field and provision of data suitablefor screening analyses. This work evaluates the potential of using available handheld, modular,and laboratory Raman spectrometers for screening normal colon tissues and benign and malignantcolon polyps. The Raman spectra of tissue samples and reference biological macromolecules weremeasured with these instruments and analyzed using curve fitting and multivariate statistics. Thespectra of calf thymus DNA measured with portable devices showed suitable signal-to-noise levelsand half-widths of the prominent bands. Band positions, resolution, and relative intensities inthe Raman spectra of colon tissues and reference compounds varied for the instruments, and thelaboratory device demonstrated the best spectral feature. The principal component analysis (PCA)of the spectra obtained with all Raman devices showed well discrimination of normal colon tissue,adenomatous polyp, and adenocarcinoma. Dendrograms of similarity obtained using hierarchycluster analysis (HCA) for the Raman spectra of all three devices also showed good separation ofthese samples. The soft independent modeling of class analogy (SIMCA) and support vector machine(SVM) models efficiently classified normal colon tissues and benign/malignant colorectal polypsbased on the Raman data from all three devices. Despite its less pronounced spectral characteristics,the handheld Raman spectrometer can be used in early diagnosis of colorectal carcinoma, comparableto the modular and laboratory instruments.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30219 - Gastroenterology and hepatology

Projekt

<a href="/cs/project/NU20-09-00229" target="_blank" >NU20-09-00229: Vývoj nových analytických přístupů pro včasné odhalení adenomatózních polypů a prevenci kolorektálního karcinomu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Applied Sciences-Basel

ISSN

2076-3417

e-ISSN

2076-3417

Svazek periodika

14

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

19

Strana od-do

—

Kód UT WoS článku

001149241300001

EID výsledku v databázi Scopus

2-s2.0-85192433337