Modelling of bubble breakage and coalescence in stirred and sparged bioreactor using the Euler–Lagrange approach coupled with volume of fluid method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43931729" target="_blank" >RIV/60461373:22340/25:43931729 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0017931025005885?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0017931025005885?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ijheatmasstransfer.2025.127249" target="_blank" >10.1016/j.ijheatmasstransfer.2025.127249</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Modelling of bubble breakage and coalescence in stirred and sparged bioreactor using the Euler–Lagrange approach coupled with volume of fluid method

Popis výsledku v původním jazyce

Efficient mass transfer is critical for stirred and sparged bioreactors used in cell culture and the production of high-value biopharmaceuticals. Accurate prediction of volumetric mass transfer coefficient kLa requires modelling of not only the dispersed bubbles but also the free surface, which is often overlooked. To address this, we developed an Euler–Lagrange (EL) model coupled with the Volume of Fluid (VOF) method, which allowed us to account for free surface dynamics and quantify its contribution to the total kLa. Since the kLa depends on the bubble size, we accounted for bubble breakage and coalescence as well as shape deformation due to turbulence. Simulations were performed for two bioreactor configurations (250 mL and 3.5 L) across a range of impeller speeds at constant gas feed rates. Results were validated against experimental data, showing strong agreement in both bubble size and kLa. The contribution of free surface to overall kLa appears to be important and comparable to the one of sparged bubbles, particularly in 250 mL reactor with very low gas flow rate. Additionally, we tested and evaluated the influence of additional forces on the results. There, an increase in void fraction, interfacial area and kLa was observed if pressure gradient force (PGF) was included. This occurred by slowing down the bubbles without significantly altering Sauter mean diameter d32. Overall, the proposed EL-VOF model provides a comprehensive and reliable framework for simulating gas-liquid systems. It accurately captures bubble behaviour, size distributions, interactions with the liquid phase and it effects on mass transfer. © 2025 Elsevier Ltd

Název v anglickém jazyce

Modelling of bubble breakage and coalescence in stirred and sparged bioreactor using the Euler–Lagrange approach coupled with volume of fluid method

Popis výsledku anglicky

Efficient mass transfer is critical for stirred and sparged bioreactors used in cell culture and the production of high-value biopharmaceuticals. Accurate prediction of volumetric mass transfer coefficient kLa requires modelling of not only the dispersed bubbles but also the free surface, which is often overlooked. To address this, we developed an Euler–Lagrange (EL) model coupled with the Volume of Fluid (VOF) method, which allowed us to account for free surface dynamics and quantify its contribution to the total kLa. Since the kLa depends on the bubble size, we accounted for bubble breakage and coalescence as well as shape deformation due to turbulence. Simulations were performed for two bioreactor configurations (250 mL and 3.5 L) across a range of impeller speeds at constant gas feed rates. Results were validated against experimental data, showing strong agreement in both bubble size and kLa. The contribution of free surface to overall kLa appears to be important and comparable to the one of sparged bubbles, particularly in 250 mL reactor with very low gas flow rate. Additionally, we tested and evaluated the influence of additional forces on the results. There, an increase in void fraction, interfacial area and kLa was observed if pressure gradient force (PGF) was included. This occurred by slowing down the bubbles without significantly altering Sauter mean diameter d32. Overall, the proposed EL-VOF model provides a comprehensive and reliable framework for simulating gas-liquid systems. It accurately captures bubble behaviour, size distributions, interactions with the liquid phase and it effects on mass transfer. © 2025 Elsevier Ltd

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20300 - Mechanical engineering

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER

ISSN

0017-9310

e-ISSN

1879-2189

Svazek periodika

252

Číslo periodika v rámci svazku

127249

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

20

Strana od-do

"nestrankovano"

Kód UT WoS článku

001523736500011

EID výsledku v databázi Scopus

2-s2.0-105009378346