SERS analysis of saliva and its key components: The effects of various collection methods, sample dilution, excitation wavelengths, and enhancing substrates

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22340%2F25%3A43933230" target="_blank" >RIV/60461373:22340/25:43933230 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0924203125000219" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0924203125000219</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.vibspec.2025.103787" target="_blank" >10.1016/j.vibspec.2025.103787</a>

Jazyk výsledku

angličtina

Název v původním jazyce

SERS analysis of saliva and its key components: The effects of various collection methods, sample dilution, excitation wavelengths, and enhancing substrates

Popis výsledku v původním jazyce

Recently, human saliva has become a subject of research as an excellent material for patient-friendly diagnostics. An increasing number of diagnostic tests utilize saliva due to its easy and noninvasive collection, eliminating the patient&apos;s stress. Simultaneously, developing Surface-Enhanced Raman Scattering (SERS) spectroscopy offers new possibilities for analyzing saliva&apos;s composition. Saliva is a complex biological material; many factors influence its composition, including medication use, diseases, stress, hormone levels, diet, age, and hydration. This complexity raises the question of whether it is possible to observe and definitively attribute changes in specific substances through SERS spectra. One of the key questions we posed is how the SERS spectrum will change with an increased level of alpha-amylase 1 A (AMY1A), an enzyme marker of acute stress. AMY1A forms complexes with proline-rich proteins (PRP). Thus, we examined whether similar spectral changes are observed with a PRP level increase in saliva. Another focus was lysozyme C (LYZ C), a nonspecific marker of infectious diseases. We examined how increased levels of LYZ C affect SERS spectra, particularly considering its sensitivity to changes in the ionic composition of saliva and its complexation with PRP and lactoferrin (LF). Moreover, we explored whether the albumin (HSA) level, which plays a vital role in regulating osmotic pressure, influences LYZ C activity and how it is manifested in SERS. Furthermore, we investigated the effect of saliva dilution and collection methods on SERS spectra. We searched for correlations with significant components such as AMY1A, HSA, LYZ C, LF, and Poly-L-proline (PLP is an analog of PRP). We showed the role of gold (Au) and silver (Ag) substrates, comparing the spectral differences. Solving the issues is crucial for the ability of SERS techniques to detect and/ or monitor biomolecules in saliva and can lead to significant advancements in noninvasive diagnostics.

Název v anglickém jazyce

SERS analysis of saliva and its key components: The effects of various collection methods, sample dilution, excitation wavelengths, and enhancing substrates

Popis výsledku anglicky

Recently, human saliva has become a subject of research as an excellent material for patient-friendly diagnostics. An increasing number of diagnostic tests utilize saliva due to its easy and noninvasive collection, eliminating the patient&apos;s stress. Simultaneously, developing Surface-Enhanced Raman Scattering (SERS) spectroscopy offers new possibilities for analyzing saliva&apos;s composition. Saliva is a complex biological material; many factors influence its composition, including medication use, diseases, stress, hormone levels, diet, age, and hydration. This complexity raises the question of whether it is possible to observe and definitively attribute changes in specific substances through SERS spectra. One of the key questions we posed is how the SERS spectrum will change with an increased level of alpha-amylase 1 A (AMY1A), an enzyme marker of acute stress. AMY1A forms complexes with proline-rich proteins (PRP). Thus, we examined whether similar spectral changes are observed with a PRP level increase in saliva. Another focus was lysozyme C (LYZ C), a nonspecific marker of infectious diseases. We examined how increased levels of LYZ C affect SERS spectra, particularly considering its sensitivity to changes in the ionic composition of saliva and its complexation with PRP and lactoferrin (LF). Moreover, we explored whether the albumin (HSA) level, which plays a vital role in regulating osmotic pressure, influences LYZ C activity and how it is manifested in SERS. Furthermore, we investigated the effect of saliva dilution and collection methods on SERS spectra. We searched for correlations with significant components such as AMY1A, HSA, LYZ C, LF, and Poly-L-proline (PLP is an analog of PRP). We showed the role of gold (Au) and silver (Ag) substrates, comparing the spectral differences. Solving the issues is crucial for the ability of SERS techniques to detect and/ or monitor biomolecules in saliva and can lead to significant advancements in noninvasive diagnostics.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10400 - Chemical sciences

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

VIBRATIONAL SPECTROSCOPY

ISSN

0924-2031

e-ISSN

1873-3697

Svazek periodika

138

Číslo periodika v rámci svazku

May

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

103787

Kód UT WoS článku

001448118000001

EID výsledku v databázi Scopus

2-s2.0-86000627964