Nuclear entry of Mason-Pfizer monkey virus core

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F60461373%3A22810%2F25%3A43932651" target="_blank" >RIV/60461373:22810/25:43932651 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60461373:22330/25:43932651

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nuclear entry of Mason-Pfizer monkey virus core

Popis výsledku v původním jazyce

Retroviruses need to deliver their genetic material into the host cell nucleus for integration. HIV‑1 (Human Immunodeficiency Virus 1) can enter the nucleus via the nuclear pore complex through active transport. Recentresearch suggests that the HIV‑1 core remains largely intact during nuclear entry, with uncoating occurring only after reaching the nucleus. However, the nuclear entry mechanism of other retroviruses remains poorly understood. Mason‑Pfizer monkey virus (MPMV), a D‑type retrovirus, serves here as a model for further understanding of retroviral nuclear entry, and offers insights into retroviral biology. We prepared GFP‑CA (green fluorescent protein – capsid protein) labeled MPMV virions, which enable a direct visualization of the retroviral core within the cell. To visualize MPMV cores post infection, we used HEK293 (Human Embryonic Kidney 293) cells expressing mScarlett‑tagged RNA helicase DHX15 (DEAH‑box helicase 15) with predominantly nuclear localization. We visualized the course of entry of GFP‑labeled MPMV capsid cores into the nucleus using confocal microscopy and live imaging. We also documented the nuclear localization of MPMV cores at various times post infection. We observed colocalization of the viral cores with DHX15‑rich spots, which are considered as splicing and transcription sites. The MPMV core nuclear entry was independent of reverse transcription as shown by the use of reverse transcription MPMV mutant. In conclusion, MPMV can enter the nucleus as an intact core. Once inside, it associates with transcriptionally active nuclear domains.Supported by the the National Institute of Virology and Bacteriology (Programme EXCELES, ID Project No. LX22NPO5103)

Název v anglickém jazyce

Nuclear entry of Mason-Pfizer monkey virus core

Popis výsledku anglicky

Retroviruses need to deliver their genetic material into the host cell nucleus for integration. HIV‑1 (Human Immunodeficiency Virus 1) can enter the nucleus via the nuclear pore complex through active transport. Recentresearch suggests that the HIV‑1 core remains largely intact during nuclear entry, with uncoating occurring only after reaching the nucleus. However, the nuclear entry mechanism of other retroviruses remains poorly understood. Mason‑Pfizer monkey virus (MPMV), a D‑type retrovirus, serves here as a model for further understanding of retroviral nuclear entry, and offers insights into retroviral biology. We prepared GFP‑CA (green fluorescent protein – capsid protein) labeled MPMV virions, which enable a direct visualization of the retroviral core within the cell. To visualize MPMV cores post infection, we used HEK293 (Human Embryonic Kidney 293) cells expressing mScarlett‑tagged RNA helicase DHX15 (DEAH‑box helicase 15) with predominantly nuclear localization. We visualized the course of entry of GFP‑labeled MPMV capsid cores into the nucleus using confocal microscopy and live imaging. We also documented the nuclear localization of MPMV cores at various times post infection. We observed colocalization of the viral cores with DHX15‑rich spots, which are considered as splicing and transcription sites. The MPMV core nuclear entry was independent of reverse transcription as shown by the use of reverse transcription MPMV mutant. In conclusion, MPMV can enter the nucleus as an intact core. Once inside, it associates with transcriptionally active nuclear domains.Supported by the the National Institute of Virology and Bacteriology (Programme EXCELES, ID Project No. LX22NPO5103)

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10607 - Virology

Projekt

<a href="/cs/project/LX22NPO5103" target="_blank" >LX22NPO5103: Národní institut virologie a bakteriologie</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů