Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F20%3A00000498" target="_blank" >RIV/61383082:_____/20:00000498 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/27283933:_____/19:00007310 RIV/65269705:_____/19:00070951

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/doi/full/10.1111/trf.15170" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1111/trf.15170</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/trf.15170" target="_blank" >10.1111/trf.15170</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method

Popis výsledku v původním jazyce

BACKGROUND: Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. METHODS: Plasma-containing products from group A and O donors were titered using the participant's routine IS one-dilution titer method. No time or temperature incubations were performed, and antihuman globulin reagent was not used. The samples were then tested using a reference method, which was a saline tube test with a 1-hour room temperature incubation; antihuman globulin was not used in the reference method. The results of the one-dilution titer were then compared to that obtained in the reference method. RESULTS: Nine centers participated in this study. There were 698 antibodies from 374 units tested by the manual IS tube one-dilution titer method; sensitivity was 0.88 (95% confidence interval [CI], 0.83–0.92), and specificity was 1.00 (95% CI, 0.98–1.00). There were 412 antibodies from 206 units tested by the manual and automated IS buffered gel card one-dilution titer method; sensitivity was 0.95 (95% CI, 0.91–0.98), and specificity was 0.87 (95% CI, 0.81–0.91). There were 98 antibodies from 49 units tested by an automated microplate IS one-dilution titer method; sensitivity was 0.76 (95% CI, 0.71–0.93), and specificity was 0.96 (95% CI, 0.92–0.99). All three methods had an accuracy rate of 90% or greater. CONCLUSION: The manual and automated one-dilution titer methods are suitable for screening plasma-containing units, although more evaluation of the automated microplate method might be required.

Název v anglickém jazyce

Comparison of titer results obtained using immediate spin one-dilution techniques to a reference method

Popis výsledku anglicky

BACKGROUND: Many transfusion services determine the titer of potentially incompatible plasma-containing products by performing a one-dilution titer at their selected titer threshold. This study compared the results of immediate spin (IS) one-dilution titers determined by three methods with a reference standard method. METHODS: Plasma-containing products from group A and O donors were titered using the participant's routine IS one-dilution titer method. No time or temperature incubations were performed, and antihuman globulin reagent was not used. The samples were then tested using a reference method, which was a saline tube test with a 1-hour room temperature incubation; antihuman globulin was not used in the reference method. The results of the one-dilution titer were then compared to that obtained in the reference method. RESULTS: Nine centers participated in this study. There were 698 antibodies from 374 units tested by the manual IS tube one-dilution titer method; sensitivity was 0.88 (95% confidence interval [CI], 0.83–0.92), and specificity was 1.00 (95% CI, 0.98–1.00). There were 412 antibodies from 206 units tested by the manual and automated IS buffered gel card one-dilution titer method; sensitivity was 0.95 (95% CI, 0.91–0.98), and specificity was 0.87 (95% CI, 0.81–0.91). There were 98 antibodies from 49 units tested by an automated microplate IS one-dilution titer method; sensitivity was 0.76 (95% CI, 0.71–0.93), and specificity was 0.96 (95% CI, 0.92–0.99). All three methods had an accuracy rate of 90% or greater. CONCLUSION: The manual and automated one-dilution titer methods are suitable for screening plasma-containing units, although more evaluation of the automated microplate method might be required.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

30205 - Hematology

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Transfusion

ISSN

0041-1132

e-ISSN

—

Svazek periodika

59

Číslo periodika v rámci svazku

Suppl 2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

6

Strana od-do

1512-1517

Kód UT WoS článku

000469482400015

EID výsledku v databázi Scopus

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