QUALITY PARAMETERS AND HEMOSTATIC FUNCTION OF LEUCODEPLEETED WHOLE BLOOD - COMPARATIVE STUDY WITH WHOLE BLOOD NON-LEUCODEPLEETED

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61383082%3A_____%2F20%3A00000992" target="_blank" >RIV/61383082:_____/20:00000992 - isvavai.cz</a>

Výsledek na webu

<a href="https://onlinelibrary.wiley.com/toc/14230410/2020/115/S1" target="_blank" >https://onlinelibrary.wiley.com/toc/14230410/2020/115/S1</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

QUALITY PARAMETERS AND HEMOSTATIC FUNCTION OF LEUCODEPLEETED WHOLE BLOOD - COMPARATIVE STUDY WITH WHOLE BLOOD NON-LEUCODEPLEETED

Popis výsledku v původním jazyce

Background: The massive bleeding and hemorrhagic shock is the 2nd most common cause of death in polytraumatic patients and the leading cause of death for young people. For resuscitation these patients is renewed interest in the use of whole blood (WB). Modern blood transfusion products should be leucodepleeted and WB should comply with this standard. Leucodepleeted WB is prepared with platelet-sparing filters, yet effect of the procedure on WB quality parameters and hemostatik function are not well characterized. Aims: The aim of the study was to evaluate in vitro quality parameters and hemostatic function of leucodepleeted WB and their comparison with conventional non-leucodepleeted WB. Methods: WB collected from 30 healthy group A donors was divided into two groups (n = 15 for each group): leucodepleeted (LWB), using in-line platelet-sparing filters and collected to blood bag system IMUFLEX® WB-SP (Terumo BCT, USA) and non-leucodepleeted WB (NLWB), collected into a standard blood bag systém CompoFlex® Single System (Fresenius Kabi, Germany). Both groups were stored at 4 _ 2°C for 14 days and following parameters were measured in days D0, D1, D3, D5, D10 and D14: WBC, RBC, PLT, HB, HCT, hemolysis, pH, TEG, FVIII, TT, PT, aPTT, aggregometry (Multiplate, Roche, Swiss), concentration of PF4 and sCD40L (ELISA). Moreover, in days D0, D7 and D14 was measured the level of expression of platelet activation marker CD62P (P-selectin) – reflecting alpha granule release, CD42b (modulation of normal platelet membrane glycoprotein), CD61 (platelet glycoproteins IIIa) and CD45 Leucocyte Common Antigen by flow cytometry. Results: No significant difference between groups LWB and NLWB was found in following parameters: PT, aPTT, Fbg, FVIII, hemolysis and HB. PLTs are at LWB significantly lower at D0 (median = 150*109/L) to compare with NLWB (median = 189*109/L). Coagulation index (CI) of TEG, is significantly different only in day D14, where is lower at LWB (median = 1,6) to compare with NLWB (median = 2,4). That correlates with different increasing concentration sCD40L in day D14. The evaluation of flow cytometry and aggregometry is not yet complete. Summary/Conclusions: The in vitro quality parameters and hemostatic function of leucodepleeted WB with platelet-sparing filter to compare with conventional nonleucodepleeted, WB are comparable and meet quality and clinical requirements. Both groups show identical or similar changes in storage time. For the final conclusion itis necess ary to wait for the evaluation of flow cytometry and aggregometry.

Název v anglickém jazyce

QUALITY PARAMETERS AND HEMOSTATIC FUNCTION OF LEUCODEPLEETED WHOLE BLOOD - COMPARATIVE STUDY WITH WHOLE BLOOD NON-LEUCODEPLEETED

Popis výsledku anglicky

Background: The massive bleeding and hemorrhagic shock is the 2nd most common cause of death in polytraumatic patients and the leading cause of death for young people. For resuscitation these patients is renewed interest in the use of whole blood (WB). Modern blood transfusion products should be leucodepleeted and WB should comply with this standard. Leucodepleeted WB is prepared with platelet-sparing filters, yet effect of the procedure on WB quality parameters and hemostatik function are not well characterized. Aims: The aim of the study was to evaluate in vitro quality parameters and hemostatic function of leucodepleeted WB and their comparison with conventional non-leucodepleeted WB. Methods: WB collected from 30 healthy group A donors was divided into two groups (n = 15 for each group): leucodepleeted (LWB), using in-line platelet-sparing filters and collected to blood bag system IMUFLEX® WB-SP (Terumo BCT, USA) and non-leucodepleeted WB (NLWB), collected into a standard blood bag systém CompoFlex® Single System (Fresenius Kabi, Germany). Both groups were stored at 4 _ 2°C for 14 days and following parameters were measured in days D0, D1, D3, D5, D10 and D14: WBC, RBC, PLT, HB, HCT, hemolysis, pH, TEG, FVIII, TT, PT, aPTT, aggregometry (Multiplate, Roche, Swiss), concentration of PF4 and sCD40L (ELISA). Moreover, in days D0, D7 and D14 was measured the level of expression of platelet activation marker CD62P (P-selectin) – reflecting alpha granule release, CD42b (modulation of normal platelet membrane glycoprotein), CD61 (platelet glycoproteins IIIa) and CD45 Leucocyte Common Antigen by flow cytometry. Results: No significant difference between groups LWB and NLWB was found in following parameters: PT, aPTT, Fbg, FVIII, hemolysis and HB. PLTs are at LWB significantly lower at D0 (median = 150*109/L) to compare with NLWB (median = 189*109/L). Coagulation index (CI) of TEG, is significantly different only in day D14, where is lower at LWB (median = 1,6) to compare with NLWB (median = 2,4). That correlates with different increasing concentration sCD40L in day D14. The evaluation of flow cytometry and aggregometry is not yet complete. Summary/Conclusions: The in vitro quality parameters and hemostatic function of leucodepleeted WB with platelet-sparing filter to compare with conventional nonleucodepleeted, WB are comparable and meet quality and clinical requirements. Both groups show identical or similar changes in storage time. For the final conclusion itis necess ary to wait for the evaluation of flow cytometry and aggregometry.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30205 - Hematology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů