Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F03%3A54030047" target="_blank" >RIV/61388955:_____/03:54030047 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389013:_____/03:54030047

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles.

Popis výsledku v původním jazyce

Amphiphilic multimolecular block copolymer micelles of polystyrene-block-poly(methacrylic acid), PS-PMA and polystyrene-block-poly(ethylene oxide), PS-PEO, were investigated by fluorescence correlation spectroscopy (FCS) in aqueous media using octadecylrhodamine B (ORB) as a micelle-specific probe, together with UV-Vis absorption and other fluorescence techniques (steady-state spectroscopy and depolarization and time-resolved fluorescence decay measurements). The aim of the study was to show that the fluorescence techniques alone provide sufficient information on the micellar system, allowing thus for the fully conclusive study. Other techniques, such as static and quasielastic light scattering (SLS and QELS) and atomic force microscopy (AFM) were usedjust for comparison in order to prove the reliability of FCS results and to estimate their accuracy. Behavior of ORB in aqueous solutions, the association-dependent changes in its UV-Vis and fluorescence spectra and its binding kinetics.

Název v anglickém jazyce

Fluorescence Correlation Spectroscopy Using Octadecylrhodamine B as a Specific Micelle-Binding Fluorescent Tag, Light Scattering and Tapping Mode Atomic Force Microscopy Studies of Amphiphilic Water-Soluble Block Copolymer Micelles.

Popis výsledku anglicky

Amphiphilic multimolecular block copolymer micelles of polystyrene-block-poly(methacrylic acid), PS-PMA and polystyrene-block-poly(ethylene oxide), PS-PEO, were investigated by fluorescence correlation spectroscopy (FCS) in aqueous media using octadecylrhodamine B (ORB) as a micelle-specific probe, together with UV-Vis absorption and other fluorescence techniques (steady-state spectroscopy and depolarization and time-resolved fluorescence decay measurements). The aim of the study was to show that the fluorescence techniques alone provide sufficient information on the micellar system, allowing thus for the fully conclusive study. Other techniques, such as static and quasielastic light scattering (SLS and QELS) and atomic force microscopy (AFM) were usedjust for comparison in order to prove the reliability of FCS results and to estimate their accuracy. Behavior of ORB in aqueous solutions, the association-dependent changes in its UV-Vis and fluorescence spectra and its binding kinetics.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Langmuir

ISSN

0743-7463

e-ISSN

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Svazek periodika

19

Číslo periodika v rámci svazku

N/A

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

4111-4119

Kód UT WoS článku

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EID výsledku v databázi Scopus

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