Povrchově závislé přechody během tvorby fosfolipidové membrány na mice, silice a sklu

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F04%3A00107576" target="_blank" >RIV/61388955:_____/04:00107576 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass

Popis výsledku v původním jazyce

Formation of supported membranes by exposure of solid surfaces to phospholipid vesicles is a much-used technique in membrane research. Freshly cleaved mica, because of its superior flatness, is a preferred support and we used ellipsometry to study membrane formation kinetics on mica. Neutral (DOPC) and negatively charged (20% DOPS / 80% DOPC) vesicles were prepared by sonication. Results were compared with membrane formation on silica and glass, and the influence of stirring, buffer, and calcium was assessed. Without calcium, PC vesicles had a low affinity (Kd = 30 ?M) for mica, and PS/PC vesicles hardly adsorbed. Addition of calcium promptly caused condensation of the adhering vesicles, with either loss of excess lipid or rapid additional lipid adsorption up to full surface coverage. Vesicle-mica interaction, rather than vesicle-vesicle interaction, seems to cause this condensation process. Membranes on mica proved unstable in Tris-HCl buffer.

Název v anglickém jazyce

Surface-Dependent Transitions during Self-Assembly of Phospholipid Membranes on Mica, Silica, and Glass

Popis výsledku anglicky

Formation of supported membranes by exposure of solid surfaces to phospholipid vesicles is a much-used technique in membrane research. Freshly cleaved mica, because of its superior flatness, is a preferred support and we used ellipsometry to study membrane formation kinetics on mica. Neutral (DOPC) and negatively charged (20% DOPS / 80% DOPC) vesicles were prepared by sonication. Results were compared with membrane formation on silica and glass, and the influence of stirring, buffer, and calcium was assessed. Without calcium, PC vesicles had a low affinity (Kd = 30 ?M) for mica, and PS/PC vesicles hardly adsorbed. Addition of calcium promptly caused condensation of the adhering vesicles, with either loss of excess lipid or rapid additional lipid adsorption up to full surface coverage. Vesicle-mica interaction, rather than vesicle-vesicle interaction, seems to cause this condensation process. Membranes on mica proved unstable in Tris-HCl buffer.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/LN00A032" target="_blank" >LN00A032: Struktura a dynamika komplexních molekulových systémů a biomolekul</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2004

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Langmuir

ISSN

0743-7463

e-ISSN

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Svazek periodika

20

Číslo periodika v rámci svazku

23

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

10129-10137

Kód UT WoS článku

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EID výsledku v databázi Scopus

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