The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F09%3A00333814" target="_blank" >RIV/61388955:_____/09:00333814 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985823:_____/09:00333814

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

Popis výsledku v původním jazyce

The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alterfluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior.

Název v anglickém jazyce

The effect of detergents on trimeric G-protein activity in isolated plasma membranes from rat brain cortex: Correlation with studies of DPH and Laurdan fluorescence

Popis výsledku anglicky

The effect of non-ionic detergents on baclofen (GABA(B)-R agonist)-stimulated G-protein activity was measured as a [S-35] GTP gamma S,IS binding assay in the plasma membranes (PM) isolated from the brain tissue. The effect was clearly biphasic - a decrease in the activity was followed by an activation maximum and finally, at high concentrations, drastic inhibition of the G-protein activity was noticed. Contrarily, specific radioligand binding to GABA(B)-receptor was inhibited in the whole range of detergent concentrations step by step, i.e. it was strictly monophasic. The magnitude of both detergent effects was decreased in the same order of potency: Brij58>Triton X-100>Digitonin. The identical order was found when comparing detergents ability to alterfluorescence anisotropy of the membrane probe 1,6-diphenyl-1,3,5-hexatriene (r (DPH)) incorporated into the hydrophobic PM interior.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

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Svazek periodika

1788

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000263794700004

EID výsledku v databázi Scopus

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