In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F09%3A00334099" target="_blank" >RIV/61388955:_____/09:00334099 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68378050:_____/09:00334099

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Popis výsledku v původním jazyce

Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA?protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA?protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA?protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using twodifferent approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA?protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA?protein complexes.

Název v anglickém jazyce

In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer

Popis výsledku anglicky

Expression of the nascent RNA transcript is regulated by its interaction with a number of proteins. Misregulation of such interactions often results in impaired cellular functions that can lead to cancer and many diseases. Our understanding of RNA?protein interaction within the cellular context is thus essential for development of novel diagnostic and therapeutic tools. While there are many in vitro methods analyzing RNA?protein interactions, in vivo approaches are scarce. Here we established a method based on fluorescence resonance energy transfer (FRET), which we termed RNA-binding mediated FRET (RB-FRET). The methods enables determination of RNA?protein interaction inside cells and was tested on hnRNP H protein binding to its cognate RNA. Using twodifferent approaches, we provided evidence that RB-FRET was sensitive enough to detect specific RNA?protein interactions in cell, providing a powerful tool to study spatial and temporal localization of specific RNA?protein complexes.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

R N A

ISSN

1355-8382

e-ISSN

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Svazek periodika

15

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

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Kód UT WoS článku

000270851000014

EID výsledku v databázi Scopus

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