Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F10%3A00345815" target="_blank" >RIV/61388955:_____/10:00345815 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

Popis výsledku v původním jazyce

Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescencecorrelation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration)making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ...

Název v anglickém jazyce

Lipid diffusion in planar membranes investigated by fluorescence correlation spectroscopy

Popis výsledku anglicky

Investigation of lipid lateral mobility in biological membranes and their artificial models provides information on membrane dynamics and structure: methods based on optical microscopy are very convenient for such investigations. We focus on fluorescencecorrelation spectroscopy (FCS), explain its principles and review its state of the art versions such as 2-focus. Z-scan or scanning FCS, which overcome most artefacts of standard FCS (especially those resulting from the need for an external calibration)making it a reliable and versatile method. FCS is also compared to single particle tracking and fluorescence photobleaching recovery and the applicability and the limitations of the methods are briefly reviewed. We discuss several key questions of lateral mobility investigation in planar lipid membranes, namely the influence which membrane and aqueous phase composition (ionic strength and sugar content), choice of a fluorescent tracer molecule, ...

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

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Svazek periodika

1798

Číslo periodika v rámci svazku

7

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

15

Strana od-do

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Kód UT WoS článku

000279365000012

EID výsledku v databázi Scopus

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