Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F12%3A00384160" target="_blank" >RIV/61388955:_____/12:00384160 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1039/c2ay25652e" target="_blank" >http://dx.doi.org/10.1039/c2ay25652e</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c2ay25652e" target="_blank" >10.1039/c2ay25652e</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions

Popis výsledku v původním jazyce

Hydrogen cyanide (HCN) and 2-aminoacetophenone (2-AA; H2NC6H4COCH3) are possible biomarkers of pulmonary Pseudomonas aeruginosa (PA) infection that could be used in an exhaled breath test. All factors affecting their production need to be investigated, including the culture conditions: planktonic (free-floating) or biofilm (non-motile communities attached to a solid surface). In vivo, the change from planktonic to biofilm growth is signalled when a certain population density is reached. Using selected ion flow tube mass spectrometry, SIFT-MS, we have analysed HCN and 2-AA produced by 12 genotyped PA samples, cultured under both planktonic and biofilm conditions after 24, 48, 72 and 96 hours of incubation. The 12 samples included 3 different strains (genotypes), 50% of which had a mucoid phenotype and 50% had a non-mucoid phenotype. All samples produced significant concentrations of HCN; median (25th to 75th percentiles, IQR) concentration: 144 (61-512) parts-per-billion by volume (ppbv

Název v anglickém jazyce

Quantification of hydrogen cyanide and 2-aminoacetophenone in the headspace of Pseudomonas aeruginosa cultured under biofilm and planktonic conditions

Popis výsledku anglicky

Hydrogen cyanide (HCN) and 2-aminoacetophenone (2-AA; H2NC6H4COCH3) are possible biomarkers of pulmonary Pseudomonas aeruginosa (PA) infection that could be used in an exhaled breath test. All factors affecting their production need to be investigated, including the culture conditions: planktonic (free-floating) or biofilm (non-motile communities attached to a solid surface). In vivo, the change from planktonic to biofilm growth is signalled when a certain population density is reached. Using selected ion flow tube mass spectrometry, SIFT-MS, we have analysed HCN and 2-AA produced by 12 genotyped PA samples, cultured under both planktonic and biofilm conditions after 24, 48, 72 and 96 hours of incubation. The 12 samples included 3 different strains (genotypes), 50% of which had a mucoid phenotype and 50% had a non-mucoid phenotype. All samples produced significant concentrations of HCN; median (25th to 75th percentiles, IQR) concentration: 144 (61-512) parts-per-billion by volume (ppbv

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

<a href="/cs/project/GA203%2F09%2F0256" target="_blank" >GA203/09/0256: Výzkum těkavých látek uvolňovaných bakteriálními kulturami metodou hmotnostní spektrometrie v proudové trubici s vybranými ionty</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods: advancing methods and applications

ISSN

1759-9660

e-ISSN

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Svazek periodika

4

Číslo periodika v rámci svazku

11

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

5

Strana od-do

3661-3665

Kód UT WoS článku

000310281700025

EID výsledku v databázi Scopus

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