Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F13%3A00382433" target="_blank" >RIV/61388955:_____/13:00382433 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/4243_2012_46" target="_blank" >http://dx.doi.org/10.1007/4243_2012_46</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/4243_2012_46" target="_blank" >10.1007/4243_2012_46</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift

Popis výsledku v původním jazyce

Biological membranes as an indispensable part of living organisms are permanently surrounded by the molecules of water. The presence of water is essential for maintaining their structure and functionality. Therefore, lipid bilayer hydration, mobility ofthe hydrated lipids, and their changes upon perturbations are appealing characteristics in the lipid membrane research. Time-dependent fluorescent shift (TDFS) measurements enable probing these properties in biologically relevant fully hydrated liquid crystalline lipid bilayers with a simple instrumentation and easy data treatment. Since the native lipid molecules do not fluoresce naturally, the extrinsic probing with a suitable fluorescent dye is necessary. There are a number of fluorescent membrane polarity probes designed for this purpose with different spectral properties and locations within the lipid bilayer. The basics of the technique are explained together with some useful additional considerations. The convenience of the TDFS

Název v anglickém jazyce

Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift

Popis výsledku anglicky

Biological membranes as an indispensable part of living organisms are permanently surrounded by the molecules of water. The presence of water is essential for maintaining their structure and functionality. Therefore, lipid bilayer hydration, mobility ofthe hydrated lipids, and their changes upon perturbations are appealing characteristics in the lipid membrane research. Time-dependent fluorescent shift (TDFS) measurements enable probing these properties in biologically relevant fully hydrated liquid crystalline lipid bilayers with a simple instrumentation and easy data treatment. Since the native lipid molecules do not fluoresce naturally, the extrinsic probing with a suitable fluorescent dye is necessary. There are a number of fluorescent membrane polarity probes designed for this purpose with different spectral properties and locations within the lipid bilayer. The basics of the technique are explained together with some useful additional considerations. The convenience of the TDFS

Druh

C - Kapitola v odborné knize

CEP obor

CF - Fyzikální chemie a teoretická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Fluorescent Methods to Study Biological Membranes

ISBN

978-3-642-33127-5

Počet stran výsledku

19

Strana od-do

141-159

Počet stran knihy

485

Název nakladatele

Springer

Místo vydání

Heidelberg

Kód UT WoS kapitoly

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