Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F15%3A00447841" target="_blank" >RIV/61388955:_____/15:00447841 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/68378050:_____/15:00447841
Výsledek na webu
<a href="http://dx.doi.org/10.1016/j.colsurfb.2015.09.012" target="_blank" >http://dx.doi.org/10.1016/j.colsurfb.2015.09.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.colsurfb.2015.09.012" target="_blank" >10.1016/j.colsurfb.2015.09.012</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics
Popis výsledku v původním jazyce
Cationic lipids are used to deliver genetic material to living cells. Their proper biophysical characterization is needed in order to design and control this process. In the present work we characterize some properties of recently synthetized cationic lipophosphoramidates. The studied compounds share the same structure of their hydrophobic backbone, but differ in their hydrophilic cationic headgroup, which is formed by a trimethylammonium, a trimethylarsonium or a dicationic moiety. Dynamic light scattering and cryo-transmission electron microscopy proves that the studied lipophosphoramidates create stable unilamellar vesicles. Fluorescence of polarity probe, Laurdan, analyzed using time-dependent fluorescence shift method (TDFS) and generalized polarization (GP) gives important information about the phase, hydration and dynamics of the lipophosphoramidate bilayers. While all of the compounds produced lipid bilayers that were sufficiently fluid for their potential application in gene t
Název v anglickém jazyce
Biophysical properties of cationic lipophosphoramidates: Vesicle morphology, bilayer hydration and dynamics
Popis výsledku anglicky
Cationic lipids are used to deliver genetic material to living cells. Their proper biophysical characterization is needed in order to design and control this process. In the present work we characterize some properties of recently synthetized cationic lipophosphoramidates. The studied compounds share the same structure of their hydrophobic backbone, but differ in their hydrophilic cationic headgroup, which is formed by a trimethylammonium, a trimethylarsonium or a dicationic moiety. Dynamic light scattering and cryo-transmission electron microscopy proves that the studied lipophosphoramidates create stable unilamellar vesicles. Fluorescence of polarity probe, Laurdan, analyzed using time-dependent fluorescence shift method (TDFS) and generalized polarization (GP) gives important information about the phase, hydration and dynamics of the lipophosphoramidate bilayers. While all of the compounds produced lipid bilayers that were sufficiently fluid for their potential application in gene t
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CF - Fyzikální chemie a teoretická chemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
<a href="/cs/project/GBP208%2F12%2FG016" target="_blank" >GBP208/12/G016: Řízení struktury a funkce biomolekul na molekulové úrovni: souhra teorie a experimentu</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2015
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Colloids and Surfaces B-Biointerfaces
ISSN
0927-7765
e-ISSN
—
Svazek periodika
136
Číslo periodika v rámci svazku
DEC 2015
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
192-200
Kód UT WoS článku
000367408100025
EID výsledku v databázi Scopus
2-s2.0-84941945136