Interaction of procyanidin B-3 with membrane lipids Fluorescence, DSC and FTIR studies

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F17%3A00476373" target="_blank" >RIV/61388955:_____/17:00476373 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.bbamem.2017.04.026" target="_blank" >http://dx.doi.org/10.1016/j.bbamem.2017.04.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bbamem.2017.04.026" target="_blank" >10.1016/j.bbamem.2017.04.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Interaction of procyanidin B-3 with membrane lipids Fluorescence, DSC and FTIR studies

Popis výsledku v původním jazyce

Procyanidins, contained in many products abundant in human diet, exhibit high biological activity. However, this activity has not been fully explained at cellular and molecular levels. In this study, we determine the mechanism of interaction of procyanidin B-3 with model lipid membrane. This mechanism was established on the basis of changes induced by B-3 in the physical properties of lipid bilayer. The changes were investigated using steady state and time-resolved fluorescence, DSC, and FTIR. We show that procyanidin B-3 causes changes in the arrangement of the polar heads of lipids, order of their acyl chains and the main lipid phase transition temperature. Furthermore, its presence in the membrane leads to a reduction in membrane dipole potential. Procyanidin B-3 is anchored to membrane via hydrogen bonds formed between its OH groups and the PO2- and C=O groups of lipids, causing changes in both hydrophilic and hydrophobic regions of the membrane. (C) 2017 Published by Elsevier B.V.

Název v anglickém jazyce

Interaction of procyanidin B-3 with membrane lipids Fluorescence, DSC and FTIR studies

Popis výsledku anglicky

Procyanidins, contained in many products abundant in human diet, exhibit high biological activity. However, this activity has not been fully explained at cellular and molecular levels. In this study, we determine the mechanism of interaction of procyanidin B-3 with model lipid membrane. This mechanism was established on the basis of changes induced by B-3 in the physical properties of lipid bilayer. The changes were investigated using steady state and time-resolved fluorescence, DSC, and FTIR. We show that procyanidin B-3 causes changes in the arrangement of the polar heads of lipids, order of their acyl chains and the main lipid phase transition temperature. Furthermore, its presence in the membrane leads to a reduction in membrane dipole potential. Procyanidin B-3 is anchored to membrane via hydrogen bonds formed between its OH groups and the PO2- and C=O groups of lipids, causing changes in both hydrophilic and hydrophobic regions of the membrane. (C) 2017 Published by Elsevier B.V.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biochimica Et Biophysica Acta-Biomembranes

ISSN

0005-2736

e-ISSN

—

Svazek periodika

1859

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

10

Strana od-do

1362-1371

Kód UT WoS článku

000403520800007

EID výsledku v databázi Scopus

2-s2.0-85019174441