Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00490126" target="_blank" >RIV/61388955:_____/18:00490126 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.026" target="_blank" >http://dx.doi.org/10.1016/j.jelechem.2018.03.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jelechem.2018.03.026" target="_blank" >10.1016/j.jelechem.2018.03.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study

Popis výsledku v původním jazyce

The ability to tune the permeability of cell membranes might have significant implications in targeted drug delivery applications. In this work we employ electrochemical methods as well as nanolithography realized in the environment of in-situ atomic force microscopy (AFM) to demonstrate that bioactive compound apigenin is capable of altering the permeability of a model phospholipid dipalmitoylphosphatidylcholine bilayer supported by a gold surface. The electron transfer rate through the bilayer was investigated employing [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox couple as an electroactive probe. The value of the standard heterogeneous rate constant obtained in the absence of apigenin (k 0 = 6.60∙10 −5 cm·s −1 ) indicates that the electron transfer through the bilayer is rather sluggish confirming its compactness. The addition of apigenin to the system leads to a considerable increase of the electron transfer rate to k 0 = 5.74∙10 −3 cm·s −1 being virtually identical to the value obtained at the bare electrode/electrolyte interface (k 0 = 2.24∙10 −3 cm·s −1 ). This suggests that apigenin induces a significant rearrangement of the supported lipid bilayer increasing thus its permeability. The AFM nanolithography performed in-situ in the presence of apigenin confirmed that the supported lipid bilayer reorganizes itself to a monolayer of molecules laying parallel to the electrode surface.

Název v anglickém jazyce

Tuning phospholipid bilayer permeability by flavonoid apigenin: Electrochemical and atomic force microscopy study

Popis výsledku anglicky

The ability to tune the permeability of cell membranes might have significant implications in targeted drug delivery applications. In this work we employ electrochemical methods as well as nanolithography realized in the environment of in-situ atomic force microscopy (AFM) to demonstrate that bioactive compound apigenin is capable of altering the permeability of a model phospholipid dipalmitoylphosphatidylcholine bilayer supported by a gold surface. The electron transfer rate through the bilayer was investigated employing [Fe(CN) 6 ] 4− /[Fe(CN) 6 ] 3− redox couple as an electroactive probe. The value of the standard heterogeneous rate constant obtained in the absence of apigenin (k 0 = 6.60∙10 −5 cm·s −1 ) indicates that the electron transfer through the bilayer is rather sluggish confirming its compactness. The addition of apigenin to the system leads to a considerable increase of the electron transfer rate to k 0 = 5.74∙10 −3 cm·s −1 being virtually identical to the value obtained at the bare electrode/electrolyte interface (k 0 = 2.24∙10 −3 cm·s −1 ). This suggests that apigenin induces a significant rearrangement of the supported lipid bilayer increasing thus its permeability. The AFM nanolithography performed in-situ in the presence of apigenin confirmed that the supported lipid bilayer reorganizes itself to a monolayer of molecules laying parallel to the electrode surface.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Electroanalytical Chemistry

ISSN

1572-6657

e-ISSN

—

Svazek periodika

821

Číslo periodika v rámci svazku

JUL 2018

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

6

Strana od-do

67-72

Kód UT WoS článku

000437818600011

EID výsledku v databázi Scopus

2-s2.0-85044052308