Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00497636" target="_blank" >RIV/61388955:_____/18:00497636 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.isci.2018.11.026" target="_blank" >http://dx.doi.org/10.1016/j.isci.2018.11.026</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.isci.2018.11.026" target="_blank" >10.1016/j.isci.2018.11.026</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

Popis výsledku v původním jazyce

The dynamics of cellular membranes is primarily determined by lipid species forming a bilayer. Proteins are considered mainly as effector molecules of diverse cellular processes. In addition to large assemblies of proteins, which were found to influence properties of fluid membranes, biological membranes are densely populated by small, highly mobile proteins. However, little is known about the effect of such proteins on the dynamics of membranes. Using synthetic peptides, we demonstrate that transmembrane helices interfere with the mobility of membrane components by trapping lipid acyl chains on their rough surfaces. The effect is more pronounced in the presence of cholesterol, which segregates from the rough surface of helical peptides. This may contribute to the formation or stabilization of membrane heterogeneities. Since roughness is a general property of helical transmembrane segments, our results suggest that, independent of their size or cytoskeleton linkage, integral membrane proteins affect local membrane dynamics and organization.

Název v anglickém jazyce

Roughness of Transmembrane Helices Reduces Lipid Membrane Dynamics

Popis výsledku anglicky

The dynamics of cellular membranes is primarily determined by lipid species forming a bilayer. Proteins are considered mainly as effector molecules of diverse cellular processes. In addition to large assemblies of proteins, which were found to influence properties of fluid membranes, biological membranes are densely populated by small, highly mobile proteins. However, little is known about the effect of such proteins on the dynamics of membranes. Using synthetic peptides, we demonstrate that transmembrane helices interfere with the mobility of membrane components by trapping lipid acyl chains on their rough surfaces. The effect is more pronounced in the presence of cholesterol, which segregates from the rough surface of helical peptides. This may contribute to the formation or stabilization of membrane heterogeneities. Since roughness is a general property of helical transmembrane segments, our results suggest that, independent of their size or cytoskeleton linkage, integral membrane proteins affect local membrane dynamics and organization.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

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OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

iScience

ISSN

2589-0042

e-ISSN

—

Svazek periodika

10

Číslo periodika v rámci svazku

DEC 2018

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

87-97

Kód UT WoS článku

000454331700008

EID výsledku v databázi Scopus

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