Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00499171" target="_blank" >RIV/61388955:_____/19:00499171 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11160/19:10399468

Výsledek na webu

<a href="http://hdl.handle.net/11104/0291428" target="_blank" >http://hdl.handle.net/11104/0291428</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dyepig.2018.10.051" target="_blank" >10.1016/j.dyepig.2018.10.051</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine

Popis výsledku v původním jazyce

We synthesized water-soluble zinc(II) complex of octa(p-sulfonatophenyl)tetrapyrazinoporphyrazine (TPyzPz), which is monomeric above pH 2.5 up to micromolar concentration. Significant aggregation occurs at lower pH as a consequence of losing repulsion forces after protonation of sulfonate groups. Changes in both absorption and fluorescence spectra indicated strong interaction of TPyzPz and bovine serum albumin (BSA) with a maximum at 3:1 ratio (dye: BSA). Binding to the BSA leads to significant quenching of the singlet excited state of the photosensitizer and to longer triplet states lifetimes as a consequence of limited diffusion of oxygen to the excited TPyzPz. The photodynamic activity of this TPyzPz was also strongly influenced by binding to BSA. It was lower in the cell culture medium containing serum (EC50 = 99.8 ± 23.5 μM) than in the medium without the proteins (EC50 = 0.83 ± 0.31 μM). The compound was of minimal dark toxicity without irradiation (TC50 > 1000 μM). Fluorescence microscopy revealed lysosomes as the localization site of the TPyzPz, their rupture after irradiation and subsequent relocalization of TPyzPz to the cytoplasm with predominantly necrotic mode of cell death.

Název v anglickém jazyce

Effect of bovine serum albumin on the photodynamic activity of sulfonated tetrapyrazinoporphyrazine

Popis výsledku anglicky

We synthesized water-soluble zinc(II) complex of octa(p-sulfonatophenyl)tetrapyrazinoporphyrazine (TPyzPz), which is monomeric above pH 2.5 up to micromolar concentration. Significant aggregation occurs at lower pH as a consequence of losing repulsion forces after protonation of sulfonate groups. Changes in both absorption and fluorescence spectra indicated strong interaction of TPyzPz and bovine serum albumin (BSA) with a maximum at 3:1 ratio (dye: BSA). Binding to the BSA leads to significant quenching of the singlet excited state of the photosensitizer and to longer triplet states lifetimes as a consequence of limited diffusion of oxygen to the excited TPyzPz. The photodynamic activity of this TPyzPz was also strongly influenced by binding to BSA. It was lower in the cell culture medium containing serum (EC50 = 99.8 ± 23.5 μM) than in the medium without the proteins (EC50 = 0.83 ± 0.31 μM). The compound was of minimal dark toxicity without irradiation (TC50 > 1000 μM). Fluorescence microscopy revealed lysosomes as the localization site of the TPyzPz, their rupture after irradiation and subsequent relocalization of TPyzPz to the cytoplasm with predominantly necrotic mode of cell death.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/EF16_019%2F0000841" target="_blank" >EF16_019/0000841: Zvýšení účinnosti a bezpečnosti léčiv a nutraceutik: moderní metody - nové výzvy</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Dyes and Pigments

ISSN

0143-7208

e-ISSN

—

Svazek periodika

162

Číslo periodika v rámci svazku

MAR 2019

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

9

Strana od-do

358-366

Kód UT WoS článku

000456226500044

EID výsledku v databázi Scopus

2-s2.0-85055714930