Markers of nucleic acids and proteins oxidative damage in acute methanol poisoning

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00503211" target="_blank" >RIV/61388955:_____/19:00503211 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10393494 RIV/61384399:31140/19:00053380 RIV/75010330:_____/19:00012601 RIV/00064165:_____/19:10393494

Výsledek na webu

<a href="http://hdl.handle.net/11104/0295033" target="_blank" >http://hdl.handle.net/11104/0295033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-2370-8" target="_blank" >10.1007/s00706-019-2370-8</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Markers of nucleic acids and proteins oxidative damage in acute methanol poisoning

Popis výsledku v původním jazyce

The aim of the study is to measure serum concentrations of markers of nucleic acids and proteins oxidative damage in humans to study the dynamics and clinical determinants of oxidative stress caused by acute methanol poisoning. Acute blood serum samples for this study were collected from 28 patients with methanol poisoning and the follow-up samples from 36 survivors of poisoning were collected 2 years after discharge. Serum concentrations of 8-hydroxy-2′-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-(hydroxymethyl)uracil (5-OHMU), ortho-tyrosine (o-Tyr), nitrotyrosine (NO-Tyr), and chlorotyrosine (Cl-Tyr) were measured by liquid chromatography-electrospray ionisation-tandem mass spectrometry. Acute concentrations of 8-OHdG and o-Tyr were significantly higher than the follow-up concentrations (94.4 ± 6.2 versus 78.0 ± 10.0 pg cm−3, p = 0.009 and 163.0 ± 11.0 versus 124.0 ± 17.0 pg cm−3, p < 0.001, correspondingly). Survivors of methanol poisoning had higher acute 8-OHdG and 8-OHG concentrations than those who died (97.3 ± 7.4 versus 50.0 ± 23.0 pg cm−3, p < 0.001 and 97.9 ± 7.2 versus 83.7 ± 6.7 pg cm−3, p = 0.047). Acute concentrations of 8-OHdG, 8-OHG, 5-OHMU, and o-Tyr were higher in the patients who survived without health sequelae than in those who survived with visual and CNS sequelae (all p < 0.05). Acute concentrations of markers of proteins and nucleic acids damage correlated with laboratory parameters of acidemia (anion gap) and serum ethanol concentration on admission (both p < 0.05). Acute elevation of the concentration of markers of nucleic acids and proteins oxidative damage in the patients with methanol poisoning suggest that mild-to-moderate oxidative stress may play an important role in the non-specific mechanisms of brain protection against direct neurotoxic effects of formic acid.

Název v anglickém jazyce

Markers of nucleic acids and proteins oxidative damage in acute methanol poisoning

Popis výsledku anglicky

The aim of the study is to measure serum concentrations of markers of nucleic acids and proteins oxidative damage in humans to study the dynamics and clinical determinants of oxidative stress caused by acute methanol poisoning. Acute blood serum samples for this study were collected from 28 patients with methanol poisoning and the follow-up samples from 36 survivors of poisoning were collected 2 years after discharge. Serum concentrations of 8-hydroxy-2′-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-(hydroxymethyl)uracil (5-OHMU), ortho-tyrosine (o-Tyr), nitrotyrosine (NO-Tyr), and chlorotyrosine (Cl-Tyr) were measured by liquid chromatography-electrospray ionisation-tandem mass spectrometry. Acute concentrations of 8-OHdG and o-Tyr were significantly higher than the follow-up concentrations (94.4 ± 6.2 versus 78.0 ± 10.0 pg cm−3, p = 0.009 and 163.0 ± 11.0 versus 124.0 ± 17.0 pg cm−3, p < 0.001, correspondingly). Survivors of methanol poisoning had higher acute 8-OHdG and 8-OHG concentrations than those who died (97.3 ± 7.4 versus 50.0 ± 23.0 pg cm−3, p < 0.001 and 97.9 ± 7.2 versus 83.7 ± 6.7 pg cm−3, p = 0.047). Acute concentrations of 8-OHdG, 8-OHG, 5-OHMU, and o-Tyr were higher in the patients who survived without health sequelae than in those who survived with visual and CNS sequelae (all p < 0.05). Acute concentrations of markers of proteins and nucleic acids damage correlated with laboratory parameters of acidemia (anion gap) and serum ethanol concentration on admission (both p < 0.05). Acute elevation of the concentration of markers of nucleic acids and proteins oxidative damage in the patients with methanol poisoning suggest that mild-to-moderate oxidative stress may play an important role in the non-specific mechanisms of brain protection against direct neurotoxic effects of formic acid.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/NV16-27075A" target="_blank" >NV16-27075A: NEURODEGENERATIVNÍ PROCESY U PACIENTŮ EXPONOVANÝCH METANOLU: PROSPEKTIVNÍ STUDIE PO HROMADNÉ OTRAVĚ METANOLEM V ČESKÉ REPUBLICE V ROCE 2012</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

11

Strana od-do

477-487

Kód UT WoS článku

000461394400015

EID výsledku v databázi Scopus

2-s2.0-85061091225