The effect of iodide and temperature on enhancing antibacterial properties of nanoparticles with an encapsulated photosensitizer

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00510021" target="_blank" >RIV/61388955:_____/19:00510021 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61388980:_____/19:00510021 RIV/00216208:11310/19:10394050

Výsledek na webu

<a href="http://hdl.handle.net/11104/0300594" target="_blank" >http://hdl.handle.net/11104/0300594</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.colsurfb.2019.01.021" target="_blank" >10.1016/j.colsurfb.2019.01.021</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The effect of iodide and temperature on enhancing antibacterial properties of nanoparticles with an encapsulated photosensitizer

Popis výsledku v původním jazyce

Aqueous dispersions of sulfonated polystyrene nanoparticles (average diameter: 30 +/- 14 nm) with encapsulated 5,10,15,20-tetraphenylporphyrin (TPP) are promising candidates for antibacterial treatments due to the photogeneration of cytotoxic singlet oxygen species O-2((1)Delta(g)) under physiological conditions using visible light. The antibacterial effect on gram-negative Escherichia coli was significantly enhanced after the addition of nontoxic potassium iodide (0.001-0.01 M) because photogenerated O-2((1)Delta(g)) oxidized iodide to I-2/I-3(-), which is another antibacterial species. The improved antibacterial properties were predicted using luminescence measurements of O-2((1)Delta(g)), transient absorption of TPP triplets and singlet oxygen-sensitized delayed fluorescence (SODF). In contrast to a solution of free photosensitizers, the aqueous dispersion of photoactive nanoparticles did not exhibit any quenching of the excited states after the addition of iodide or any tendency toward aggregation and/or I-3(-)-induced photo-aggregation. We also observed a decrease in the lifetime of O-2((1)Delta(g)) and a significant increase in SODF intensity at higher temperatures, due to the increased oxygen diffusion coefficient in nanoparticles and aqueous surroundings. This effect corresponds with the significantly stronger antibacterial effect of nano particles at physiological temperature (37 degrees C) in comparison with that at room temperature (25 degrees C).

Název v anglickém jazyce

The effect of iodide and temperature on enhancing antibacterial properties of nanoparticles with an encapsulated photosensitizer

Popis výsledku anglicky

Aqueous dispersions of sulfonated polystyrene nanoparticles (average diameter: 30 +/- 14 nm) with encapsulated 5,10,15,20-tetraphenylporphyrin (TPP) are promising candidates for antibacterial treatments due to the photogeneration of cytotoxic singlet oxygen species O-2((1)Delta(g)) under physiological conditions using visible light. The antibacterial effect on gram-negative Escherichia coli was significantly enhanced after the addition of nontoxic potassium iodide (0.001-0.01 M) because photogenerated O-2((1)Delta(g)) oxidized iodide to I-2/I-3(-), which is another antibacterial species. The improved antibacterial properties were predicted using luminescence measurements of O-2((1)Delta(g)), transient absorption of TPP triplets and singlet oxygen-sensitized delayed fluorescence (SODF). In contrast to a solution of free photosensitizers, the aqueous dispersion of photoactive nanoparticles did not exhibit any quenching of the excited states after the addition of iodide or any tendency toward aggregation and/or I-3(-)-induced photo-aggregation. We also observed a decrease in the lifetime of O-2((1)Delta(g)) and a significant increase in SODF intensity at higher temperatures, due to the increased oxygen diffusion coefficient in nanoparticles and aqueous surroundings. This effect corresponds with the significantly stronger antibacterial effect of nano particles at physiological temperature (37 degrees C) in comparison with that at room temperature (25 degrees C).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GA16-15020S" target="_blank" >GA16-15020S: Fotoaktivní polymerní materiály s nanostrukturovanými povrchy pro biomedicínské aplikace</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Colloids and Surfaces B-Biointerfaces

ISSN

0927-7765

e-ISSN

—

Svazek periodika

176

Číslo periodika v rámci svazku

APR 2019

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

334-340

Kód UT WoS článku

000465051800038

EID výsledku v databázi Scopus

2-s2.0-85059834822