Can proteomics predict the prognosis in chronic dioxin intoxication?

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F19%3A00517165" target="_blank" >RIV/61388955:_____/19:00517165 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11110/19:10400450 RIV/00216208:11310/19:10400450 RIV/00064165:_____/19:10400450

Výsledek na webu

<a href="http://hdl.handle.net/11104/0302452" target="_blank" >http://hdl.handle.net/11104/0302452</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00706-019-02460-0" target="_blank" >10.1007/s00706-019-02460-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Can proteomics predict the prognosis in chronic dioxin intoxication?

Popis výsledku v původním jazyce

Untargeted proteomic analysis of exhaled breath condensate (EBC) was used to estimate the effects and prognosis of chronic intoxication with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to elucidate the mechanisms involved. EBC was collected from eight males (72.4 +/- 1.3 years) exposed to TCDD in 1965-1968 and seven control males (66 +/- 16 years). The median TCDD blood level was 112 (46-390) ng/kg fat in the patients 50 years after exposure and 12 ng/kg in the controls. The shotgun method for identifying proteins was performed after proteolytic digestion and high-performance liquid chromatography combined with mass spectrometry. Raw data were evaluated using the MaxQuant software and protein atlas. A total of 332 proteins were detected: one protein was unique to TCDD patients and 11 to the controls. These proteins may have come from many organs, in addition to the lungs and bronchi. The salivary contribution based on alpha amylase proportion to the overall protein's intensity was only 0.003%. The intensity of nine proteins was correlated with TCDD plasma level (two positively, seven negatively), but not with alpha amylase. This excludes saliva contamination, as this enzyme is the marker of salivary contamination. Several of the changes in intensity were associated with an unfavourable prognosis of cancers. Proteomics of EBC may bring new information and point to the mechanisms by which this highly toxic chemical and human carcinogen potentially affects any organ.

Název v anglickém jazyce

Can proteomics predict the prognosis in chronic dioxin intoxication?

Popis výsledku anglicky

Untargeted proteomic analysis of exhaled breath condensate (EBC) was used to estimate the effects and prognosis of chronic intoxication with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to elucidate the mechanisms involved. EBC was collected from eight males (72.4 +/- 1.3 years) exposed to TCDD in 1965-1968 and seven control males (66 +/- 16 years). The median TCDD blood level was 112 (46-390) ng/kg fat in the patients 50 years after exposure and 12 ng/kg in the controls. The shotgun method for identifying proteins was performed after proteolytic digestion and high-performance liquid chromatography combined with mass spectrometry. Raw data were evaluated using the MaxQuant software and protein atlas. A total of 332 proteins were detected: one protein was unique to TCDD patients and 11 to the controls. These proteins may have come from many organs, in addition to the lungs and bronchi. The salivary contribution based on alpha amylase proportion to the overall protein's intensity was only 0.003%. The intensity of nine proteins was correlated with TCDD plasma level (two positively, seven negatively), but not with alpha amylase. This excludes saliva contamination, as this enzyme is the marker of salivary contamination. Several of the changes in intensity were associated with an unfavourable prognosis of cancers. Proteomics of EBC may bring new information and point to the mechanisms by which this highly toxic chemical and human carcinogen potentially affects any organ.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Monatshefte fur Chemie

ISSN

0026-9247

e-ISSN

—

Svazek periodika

150

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

AT - Rakouská republika

Počet stran výsledku

8

Strana od-do

1715-1722

Kód UT WoS článku

000482907600023

EID výsledku v databázi Scopus

2-s2.0-85070323932