Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00532424" target="_blank" >RIV/61388955:_____/21:00532424 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/21:10417793

Výsledek na webu

<a href="http://hdl.handle.net/11104/0310920" target="_blank" >http://hdl.handle.net/11104/0310920</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2020.121594" target="_blank" >10.1016/j.talanta.2020.121594</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction

Popis výsledku v původním jazyce

Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5).

Název v anglickém jazyce

Differential pulse voltammetric determination of homovanillic acid as a tumor biomarker in human urine after hollow fiber-based liquid-phase microextraction

Popis výsledku anglicky

Novel method for the determination of a tumor marker homovanillic acid (HVA) in human urine was developed. Combination of hollow fiber – based liquid-phase microextraction (HF-LPME) and differential pulse voltammetry (DPV) at a cathodically pre-treated boron doped diamond electrode (BDDE) was applied for these purposes. Optimum conditions were: butyl benzoate as supported liquid membrane (SLM) formed on polypropylene HF, 0.1 mol L−1 HCl as donor phase, 0.1 mol L−1 sodium phosphate buffer of pH 6 as acceptor phase, and 30 min extraction time. HF-LPME-DPV concentration dependence was linear in the range from 1.2 to 100 μmol L−1. Limits of quantification (LOQ) and detection (LOD) were 1.2 and 0.4 μmol L−1, respectively. The applicability of the developed method was verified by analysis of human urine. Standard addition method was used, found HVA concentration was 13.5 ± 1.3 μmol L−1, RSD = 9.3% (n=5).

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Projekt

<a href="/cs/project/GA20-01589S" target="_blank" >GA20-01589S: Nové strategie pro zlepšení senzorových vlastností nových elektrodových materiálů prostřednictvím jejich předúpravy či modifikace povrchu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Svazek periodika

221

Číslo periodika v rámci svazku

JAN 2021

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

121594

Kód UT WoS článku

000579910600084

EID výsledku v databázi Scopus

2-s2.0-85091253335