Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F21%3A00543208" target="_blank" >RIV/61388955:_____/21:00543208 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11310/21:10429959

Výsledek na webu

<a href="http://hdl.handle.net/11104/0320478" target="_blank" >http://hdl.handle.net/11104/0320478</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2021.130252" target="_blank" >10.1016/j.snb.2021.130252</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization

Popis výsledku v původním jazyce

This is the first report of a novel strategy for uric acid (UA) determination via newly developed highly stable flow biosensors based on different enzymatic mini-reactors in combination with a four-electron reduction of the consumed oxygen. The design of the biosensors provides a spatial separation of the detection (working electrode) and biorecognition (uricase-based mini-reactor) part. To prepare the most efficient enzymatic mini-reactor, a comparative study focused on three different techniques of the covalent uricase immobilization at two kinds of mesoporous silica powders (SBA−15 and MCM−41) and the physical enzyme adsorption at four types of carbonaceous powders (glassy carbon, graphite, Starbon® 300, and Starbon® 800) has been performed. All investigations were carried out for two kinds of uricase: from Candida sp. (UOx(c)) and from Bacillus fastidiosus (UOx(b)) to estimate the influence of uricase source. The flow-through cell with tubular detector of silver solid amalgam was used for amperometric monitoring of four-electron oxygen reduction at the highly negative potential (−1100 mV vs. SCE). It was found that the developed UA biosensors responses are strongly dependent on the enzyme immobilization strategy. UOx(c) bounded to glassy carbon by physical adsorption has provided good results in terms of sensitivity, stability, and repeatability for UA determination. The biosensor with the mini-reactor based on the covalent immobilization of UOx(b) at glutaraldehyde activated single bondNH2 functionalized MCM–41 has shown extremely high stability (more than 1 year) and reusability (at least 600 measurements) The biosensor’s practical applicability has been tested by successful determination of UA in human urine.

Název v anglickém jazyce

Flow amperometric uric acid biosensors based on different enzymatic mini-reactors: A comparative study of uricase immobilization

Popis výsledku anglicky

This is the first report of a novel strategy for uric acid (UA) determination via newly developed highly stable flow biosensors based on different enzymatic mini-reactors in combination with a four-electron reduction of the consumed oxygen. The design of the biosensors provides a spatial separation of the detection (working electrode) and biorecognition (uricase-based mini-reactor) part. To prepare the most efficient enzymatic mini-reactor, a comparative study focused on three different techniques of the covalent uricase immobilization at two kinds of mesoporous silica powders (SBA−15 and MCM−41) and the physical enzyme adsorption at four types of carbonaceous powders (glassy carbon, graphite, Starbon® 300, and Starbon® 800) has been performed. All investigations were carried out for two kinds of uricase: from Candida sp. (UOx(c)) and from Bacillus fastidiosus (UOx(b)) to estimate the influence of uricase source. The flow-through cell with tubular detector of silver solid amalgam was used for amperometric monitoring of four-electron oxygen reduction at the highly negative potential (−1100 mV vs. SCE). It was found that the developed UA biosensors responses are strongly dependent on the enzyme immobilization strategy. UOx(c) bounded to glassy carbon by physical adsorption has provided good results in terms of sensitivity, stability, and repeatability for UA determination. The biosensor with the mini-reactor based on the covalent immobilization of UOx(b) at glutaraldehyde activated single bondNH2 functionalized MCM–41 has shown extremely high stability (more than 1 year) and reusability (at least 600 measurements) The biosensor’s practical applicability has been tested by successful determination of UA in human urine.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

<a href="/cs/project/GA20-07350S" target="_blank" >GA20-07350S: Elektrochemické a analytické aspekty transportu návykových a psychotropních látek přes modelové biologické bariery</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B - Chemical

ISSN

0925-4005

e-ISSN

0925-4005

Svazek periodika

344

Číslo periodika v rámci svazku

OCT 2021

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

13

Strana od-do

130252

Kód UT WoS článku

000679299600005

EID výsledku v databázi Scopus

2-s2.0-85107759684