Bovine Serum Albumin Adsorption at a Polarized Water/1,2-Dichloroethane Interface with No Effect on the Ion Transfer Kinetics

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F22%3A00558478" target="_blank" >RIV/61388955:_____/22:00558478 - isvavai.cz</a>

Výsledek na webu

<a href="http://hdl.handle.net/11104/0332125" target="_blank" >http://hdl.handle.net/11104/0332125</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/celc.202200409" target="_blank" >10.1002/celc.202200409</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Bovine Serum Albumin Adsorption at a Polarized Water/1,2-Dichloroethane Interface with No Effect on the Ion Transfer Kinetics

Popis výsledku v původním jazyce

Adsorption of bovine serum albumin (BSA) at a polarized water/1,2-dichloroethane (DCE) interface is examined by measuring the dynamic interfacial tension gamma at various cell potentials E, BSA concentrations and pH of the aqueous phase (w). An addition of BSA to the phase (w) results in an appreciable decay of gamma with time in the whole potential range available, which follows an induction period at the very beginning of the gamma transient. An analysis of the effect of the BSA concentration on the gamma transients enables to establish the adsorption isotherm, and to evaluate both the maximum surface excess concentration of BSA and the standard Gibbs energy of adsorption. An increase of the potential E results in an acceleration of the adsorption, and an increase of the surface excess concentration of the positively charged BSA at pH 2, while the latter effect is absent when BSA is charged negatively at pH 7.3. It is shown that the adsorbed BSA has a negligible effect on both the interfacial capacitance of the polarized water/DCE interface and the rate constant of the interfacial transfer of the tetraethylammonium (TEA(+)) or Cs+ ion measured at the corresponding standard ion transfer potential.

Název v anglickém jazyce

Bovine Serum Albumin Adsorption at a Polarized Water/1,2-Dichloroethane Interface with No Effect on the Ion Transfer Kinetics

Popis výsledku anglicky

Adsorption of bovine serum albumin (BSA) at a polarized water/1,2-dichloroethane (DCE) interface is examined by measuring the dynamic interfacial tension gamma at various cell potentials E, BSA concentrations and pH of the aqueous phase (w). An addition of BSA to the phase (w) results in an appreciable decay of gamma with time in the whole potential range available, which follows an induction period at the very beginning of the gamma transient. An analysis of the effect of the BSA concentration on the gamma transients enables to establish the adsorption isotherm, and to evaluate both the maximum surface excess concentration of BSA and the standard Gibbs energy of adsorption. An increase of the potential E results in an acceleration of the adsorption, and an increase of the surface excess concentration of the positively charged BSA at pH 2, while the latter effect is absent when BSA is charged negatively at pH 7.3. It is shown that the adsorbed BSA has a negligible effect on both the interfacial capacitance of the polarized water/DCE interface and the rate constant of the interfacial transfer of the tetraethylammonium (TEA(+)) or Cs+ ion measured at the corresponding standard ion transfer potential.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10403 - Physical chemistry

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ChemElectroChem

ISSN

2196-0216

e-ISSN

2196-0216

Svazek periodika

9

Číslo periodika v rámci svazku

13

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

9

Strana od-do

e202200409

Kód UT WoS článku

000810206300001

EID výsledku v databázi Scopus

2-s2.0-85131520144