Influence of BAKs on tear film lipid layer: In vitro and in silico models
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00570868" target="_blank" >RIV/61388955:_____/23:00570868 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216208:11310/23:10466258
Výsledek na webu
<a href="https://hdl.handle.net/11104/0342196" target="_blank" >https://hdl.handle.net/11104/0342196</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ejpb.2023.03.007" target="_blank" >10.1016/j.ejpb.2023.03.007</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Influence of BAKs on tear film lipid layer: In vitro and in silico models
Popis výsledku v původním jazyce
Benzalkonium chloride (BAK) compounds are commonly used in topical ophthalmic products as preservatives and stabilizers. BAK mixtures containing several compounds with different alkyl chain lengths are typically used. However, in chronic eye conditions, such as dry eye disease and glaucoma, the accumulation of adverse effects of BAKs was observed. Hence, preservative-free eye drops formulations are preferred. On the other hand, selected long-chain BAKs, particularly cetalkonium chloride, exhibit therapeutic functions, promoting epithelium wound healing and tear film stability. Nevertheless, the mechanism of BAKs influence on the tear film is not fully understood. By employing in vitro experimental and in silico simulation techniques, we elucidate the action of BAKs and demonstrate that long-chain BAKs accumulate in the lipid layer of the tear film model, stabilizing it in a concentration-dependent fashion. In contrast, short-chain BAKs interacting with the lipid layer compromise the tear film model stability. These findings are relevant for topical ophthalmic drug formulation and delivery in the context of selecting proper BAK species and understanding the dose dependency for tear film stability.
Název v anglickém jazyce
Influence of BAKs on tear film lipid layer: In vitro and in silico models
Popis výsledku anglicky
Benzalkonium chloride (BAK) compounds are commonly used in topical ophthalmic products as preservatives and stabilizers. BAK mixtures containing several compounds with different alkyl chain lengths are typically used. However, in chronic eye conditions, such as dry eye disease and glaucoma, the accumulation of adverse effects of BAKs was observed. Hence, preservative-free eye drops formulations are preferred. On the other hand, selected long-chain BAKs, particularly cetalkonium chloride, exhibit therapeutic functions, promoting epithelium wound healing and tear film stability. Nevertheless, the mechanism of BAKs influence on the tear film is not fully understood. By employing in vitro experimental and in silico simulation techniques, we elucidate the action of BAKs and demonstrate that long-chain BAKs accumulate in the lipid layer of the tear film model, stabilizing it in a concentration-dependent fashion. In contrast, short-chain BAKs interacting with the lipid layer compromise the tear film model stability. These findings are relevant for topical ophthalmic drug formulation and delivery in the context of selecting proper BAK species and understanding the dose dependency for tear film stability.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
10403 - Physical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA21-19854S" target="_blank" >GA21-19854S: Lipidové multivrstvy v biologickém kontextu - mikroskopie Langmuirova filmu a simulace molekulové dynamiky</a><br>
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2023
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
European Journal of Pharmaceutics and Biopharmaceutics
ISSN
0939-6411
e-ISSN
1873-3441
Svazek periodika
186
Číslo periodika v rámci svazku
MAY 2023
Stát vydavatele periodika
NL - Nizozemsko
Počet stran výsledku
9
Strana od-do
65-73
Kód UT WoS článku
000971535000001
EID výsledku v databázi Scopus
2-s2.0-85151022782