Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F23%3A00574988" target="_blank" >RIV/61388955:_____/23:00574988 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/67985882:_____/23:00574988

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.snb.2023.133676" target="_blank" >http://dx.doi.org/10.1016/j.snb.2023.133676</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2023.133676" target="_blank" >10.1016/j.snb.2023.133676</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field

Popis výsledku v původním jazyce

Pulsed electric field (PEF) technology is an important emerging modality for both biomedicine and the food industry, yet the mechanisms of action of a PEF on a biomolecular level remain unclear. In order to gain a better understanding of this, a sensing platform was developed to enable sensitive detection of oxidative processes induced by the PEF. It was found that the application of PEF in phosphate buffer leads to the electrogeneration of reactive oxygen species (ROS) which in turn triggers intrinsic chemiluminescence. Furthermore, the presence of the bovine serum albumin (BSA) was found to increase the luminescence signal, which was attributed to biochemiluminescence due to the reactions of BSA with the electrogenerated ROS. A carbonyl determination assay was then used to confirm that the BSA molecules had indeed been oxidatively modified. We proposed a scheme describing the reactions leading from charge transfer at the anode/electrolyte interface to the actual photon emission, helping to elucidate the mechanisms of action of PEF on proteins. The results may to open up new avenues for the application of PEF technology.

Název v anglickém jazyce

Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field

Popis výsledku anglicky

Pulsed electric field (PEF) technology is an important emerging modality for both biomedicine and the food industry, yet the mechanisms of action of a PEF on a biomolecular level remain unclear. In order to gain a better understanding of this, a sensing platform was developed to enable sensitive detection of oxidative processes induced by the PEF. It was found that the application of PEF in phosphate buffer leads to the electrogeneration of reactive oxygen species (ROS) which in turn triggers intrinsic chemiluminescence. Furthermore, the presence of the bovine serum albumin (BSA) was found to increase the luminescence signal, which was attributed to biochemiluminescence due to the reactions of BSA with the electrogenerated ROS. A carbonyl determination assay was then used to confirm that the BSA molecules had indeed been oxidatively modified. We proposed a scheme describing the reactions leading from charge transfer at the anode/electrolyte interface to the actual photon emission, helping to elucidate the mechanisms of action of PEF on proteins. The results may to open up new avenues for the application of PEF technology.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

20401 - Chemical engineering (plants, products)

Projekt

<a href="/cs/project/GX20-06873X" target="_blank" >GX20-06873X: SubTHz chipová zařízení pro řízení proteinových nanopřístrojů</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Sensors and Actuators B - Chemical

ISSN

0925-4005

e-ISSN

0925-4005

Svazek periodika

385

Číslo periodika v rámci svazku

JUN 15

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

11

Strana od-do

133676

Kód UT WoS článku

000982222600001

EID výsledku v databázi Scopus

2-s2.0-85150920169