Synthetické peptidy odvozené od Fasciola gigantica cathepsin L proteinasy a jejich využití pro imunodiagnostiku a prevenci fasciolosy u ovcí

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F08%3A00307258" target="_blank" >RIV/61388963:_____/08:00307258 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

Popis výsledku v původním jazyce

We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with Fasciola gigantica following immunization with peptides based on the H-Asp110-Lys-Ile-Asp-Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val123-OH (Fas14p) sequence of F. gigantica cathepsin L-cysteine proteinase. This sequence was synthesized as 1) N .alpha. acetylated (FasAc14p), 2) bearing at the aminoterminus an N .alpha.-acetylated cystein (FasAcCys14p), and 3) conjugated in four copies tosequential oligopeptide carrier Ac-[Lys-Aib-Gly]4-OH (Ac-SOC4) through an amide bond (Ac-[Lys(Fas14p)-Aib-Gly]4-OH). The peptides were synthesized manually by SPPS on a Wang resin using the Fmoc/tBu strategy. All compounds were tested as immunodiagnostics for sheep fasciolosis. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with F. gigantica.

Název v anglickém jazyce

Fasciola gigantica cathepsin L proteinase-based synthetic peptide for immunodiagnosis and prevention of sheep fasciolosis

Popis výsledku anglicky

We herein report on immunodiagnosis of fasciolosis, and significant protection of sheep against challenge infection with Fasciola gigantica following immunization with peptides based on the H-Asp110-Lys-Ile-Asp-Trp-Arg-Glu-Ser-Gly-Tyr-Val-Thr-Glu-Val123-OH (Fas14p) sequence of F. gigantica cathepsin L-cysteine proteinase. This sequence was synthesized as 1) N .alpha. acetylated (FasAc14p), 2) bearing at the aminoterminus an N .alpha.-acetylated cystein (FasAcCys14p), and 3) conjugated in four copies tosequential oligopeptide carrier Ac-[Lys-Aib-Gly]4-OH (Ac-SOC4) through an amide bond (Ac-[Lys(Fas14p)-Aib-Gly]4-OH). The peptides were synthesized manually by SPPS on a Wang resin using the Fmoc/tBu strategy. All compounds were tested as immunodiagnostics for sheep fasciolosis. The data together indicated that the peptides might be of considerable use for discriminating between early and late, and low and high burden, sheep infection with F. gigantica.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biopolymers

ISSN

0006-3525

e-ISSN

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Svazek periodika

90

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

349-357

Kód UT WoS článku

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EID výsledku v databázi Scopus

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