Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00378945" target="_blank" >RIV/61388963:_____/12:00378945 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00027162:_____/12:#0000885 RIV/60461373:22330/12:43894051

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.jconrel.2012.01.040" target="_blank" >http://dx.doi.org/10.1016/j.jconrel.2012.01.040</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.jconrel.2012.01.040" target="_blank" >10.1016/j.jconrel.2012.01.040</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

Popis výsledku v původním jazyce

We designed and synthesised a series of new cationic lipids based on spermine linked to various hydrophobic anchors. These lipids could be potentially useful for the preparation of stable cationic liposomes intended for the construction of drug targetingsystems applicable in the field of anticancer/antiviral therapy, vaccine carriers, and vectors for the gene therapy. Low in vitro toxicity was found for these compounds, especially for LD1, in several cell lines. The delivery of both a fluorescence marker (calcein) and antiviral drugs into cells has been achieved owing to a large extent of internalization of cationic liposomes (labelled by Lyssamine-Rhodamine PE or fluorescein-PE) as demonstrated by fluorescent microscopy and quantified by flow cytometry. The bovine herpes virus type 1 (BHV-1) virus infection in vitromodel using MDBK cells was employed to study the effect of the established antiviral drug HPMPC (Cidofovir (R)) developed by Prof. A. Holy. Inhibition of BHV-1 virus repli

Název v anglickém jazyce

Antiviral effect of HPMPC (Cidofovir (R)), entrapped in cationic liposomes: In vitro study on MDBK cell and BHV-1 virus

Popis výsledku anglicky

We designed and synthesised a series of new cationic lipids based on spermine linked to various hydrophobic anchors. These lipids could be potentially useful for the preparation of stable cationic liposomes intended for the construction of drug targetingsystems applicable in the field of anticancer/antiviral therapy, vaccine carriers, and vectors for the gene therapy. Low in vitro toxicity was found for these compounds, especially for LD1, in several cell lines. The delivery of both a fluorescence marker (calcein) and antiviral drugs into cells has been achieved owing to a large extent of internalization of cationic liposomes (labelled by Lyssamine-Rhodamine PE or fluorescein-PE) as demonstrated by fluorescent microscopy and quantified by flow cytometry. The bovine herpes virus type 1 (BHV-1) virus infection in vitromodel using MDBK cells was employed to study the effect of the established antiviral drug HPMPC (Cidofovir (R)) developed by Prof. A. Holy. Inhibition of BHV-1 virus repli

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Controlled Release

ISSN

0168-3659

e-ISSN

—

Svazek periodika

160

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

9

Strana od-do

330-338

Kód UT WoS článku

000305788800027

EID výsledku v databázi Scopus

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