Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00380954" target="_blank" >RIV/61388963:_____/12:00380954 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.chroma.2012.04.055" target="_blank" >http://dx.doi.org/10.1016/j.chroma.2012.04.055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.chroma.2012.04.055" target="_blank" >10.1016/j.chroma.2012.04.055</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry

Popis výsledku v původním jazyce

Fatty acid methyl esters (FAMEs) were analysed by reversed-phase HPLC coupled with atmospheric pressure chemical ionisation (APCI) mass spectrometry. The chromatographic separations of the FAMEs were optimised using acetonitrile or binary acetonitrile gradients and C18 or C30 columns. The gas-phase reactions of acetonitrile and unsaturated FAMEs in the APCI source provided [M+C3H5N]+ adducts. When fragmented, these adducts yielded diagnostic ions, allowing the unambiguous localisation of double bonds. The formation and fragmentation of the acetonitrile-related adduct was utilised for the structural characterisation of the FAMEs separated by HPLC. The APCI-MS detection of FAMEs encompassed a full-spectrum scan (providing information on the number of carbons and double bonds) and a data-dependent MS/MS scan of the [M+C3H5N]+ ions (the position of the double bonds). The utility of this approach was demonstrated using a mixture of FAMEs from blackcurrant-seed oil. All the unsaturated fatty

Název v anglickém jazyce

Identification of the double-bond position in fatty acid methyl esters by liquid chromatography/atmospheric pressure chemical ionisation mass spectrometry

Popis výsledku anglicky

Fatty acid methyl esters (FAMEs) were analysed by reversed-phase HPLC coupled with atmospheric pressure chemical ionisation (APCI) mass spectrometry. The chromatographic separations of the FAMEs were optimised using acetonitrile or binary acetonitrile gradients and C18 or C30 columns. The gas-phase reactions of acetonitrile and unsaturated FAMEs in the APCI source provided [M+C3H5N]+ adducts. When fragmented, these adducts yielded diagnostic ions, allowing the unambiguous localisation of double bonds. The formation and fragmentation of the acetonitrile-related adduct was utilised for the structural characterisation of the FAMEs separated by HPLC. The APCI-MS detection of FAMEs encompassed a full-spectrum scan (providing information on the number of carbons and double bonds) and a data-dependent MS/MS scan of the [M+C3H5N]+ ions (the position of the double bonds). The utility of this approach was demonstrated using a mixture of FAMEs from blackcurrant-seed oil. All the unsaturated fatty

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA203%2F09%2F0139" target="_blank" >GA203/09/0139: Komplexní lipidomická charakterizace rostlinných a živočišných tkání</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Chromatography A

ISSN

0021-9673

e-ISSN

—

Svazek periodika

1259

Číslo periodika v rámci svazku

12 Oct

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

7

Strana od-do

244-250

Kód UT WoS článku

000309566500023

EID výsledku v databázi Scopus

—