Atomic Scissors: A New Method of Tracking the 5-Bromo-2-Deoxyuridine-Labeled DNA In Situ

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F12%3A00386203" target="_blank" >RIV/61388963:_____/12:00386203 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/12:00386203 RIV/68378050:_____/12:00386203

Výsledek na webu

<a href="http://dx.doi.org/10.1371/journal.pone.0052584" target="_blank" >http://dx.doi.org/10.1371/journal.pone.0052584</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0052584" target="_blank" >10.1371/journal.pone.0052584</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Atomic Scissors: A New Method of Tracking the 5-Bromo-2-Deoxyuridine-Labeled DNA In Situ

Popis výsledku v původním jazyce

A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from deoxyribose culminating in the elimination of the nucleobase, scission of the nucleic-acid strand and formation of frequent gaps. The gaps allow the reaction of the antibodies with the commonly used markers of replication (e.g. 5-bromo-2-deoxyuridine), which are otherwise masked. The method developed makes it possible to detect nuclear and mitochondrial DNA replication efficiently. In most cases, it does not inhibit effective protein detections and in addition enables simultaneous localization of newly-synthesized RNA. The alternative presently-used methods result in protein denaturation and/or extensive DNA cleavage followed by the DNA-bound proteins peeling off.

Název v anglickém jazyce

Atomic Scissors: A New Method of Tracking the 5-Bromo-2-Deoxyuridine-Labeled DNA In Situ

Popis výsledku anglicky

A new method of the light microscopy detection of BrdU-labeled DNA in situ is described. It is based on the oxidative attack at the deoxyribose moiety by copper(I) in the presence of oxygen, which leads to the abstraction of hydrogen atom from deoxyribose culminating in the elimination of the nucleobase, scission of the nucleic-acid strand and formation of frequent gaps. The gaps allow the reaction of the antibodies with the commonly used markers of replication (e.g. 5-bromo-2-deoxyuridine), which are otherwise masked. The method developed makes it possible to detect nuclear and mitochondrial DNA replication efficiently. In most cases, it does not inhibit effective protein detections and in addition enables simultaneous localization of newly-synthesized RNA. The alternative presently-used methods result in protein denaturation and/or extensive DNA cleavage followed by the DNA-bound proteins peeling off.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

—

Projekt

<a href="/cs/project/KAN200520801" target="_blank" >KAN200520801: Cílená exprese a transport bioaktivních molekul</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2012

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PLoS ONE

ISSN

1932-6203

e-ISSN

—

Svazek periodika

7

Číslo periodika v rámci svazku

12

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000313618800107

EID výsledku v databázi Scopus

—