New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00391965" target="_blank" >RIV/61388963:_____/13:00391965 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.peptides.2012.09.033" target="_blank" >http://dx.doi.org/10.1016/j.peptides.2012.09.033</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.peptides.2012.09.033" target="_blank" >10.1016/j.peptides.2012.09.033</a>

Jazyk výsledku

angličtina

Název v původním jazyce

New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges

Popis výsledku v původním jazyce

The CART (cocaine- and amphetamine-regulated transcript) peptide is an anorexigenic neuropeptide that acts in the hypothalamus. The receptor and the mechanism of action of this peptide are still unknown. In our previous study, we showed that the CART peptide binds specifically to PC12 rat pheochromocytoma cells in both the native and differentiated into neuronal phenotype. Two biologically active forms, CART(55-102) and CART(61-102), with equal biological activity, contain three disulfide bridges. To clarify the importance of each of these disulfide bridges in maintaining the biological activity of CART(61-102), an Ala scan at particular S-S bridges forming cysteines was performed, and analogs with only one or two disulfide bridges were synthesized. Inthis study, a stabilized CART(61-102) analog with norleucine instead of methionine at position 67 was also prepared and was found to bind to PC12 cells with an anorexigenic potency similar to that of CART(61-102). The binding study revea

Název v anglickém jazyce

New analogs of the CART peptide with anorexigenic potency: The importance of individual disulfide bridges

Popis výsledku anglicky

The CART (cocaine- and amphetamine-regulated transcript) peptide is an anorexigenic neuropeptide that acts in the hypothalamus. The receptor and the mechanism of action of this peptide are still unknown. In our previous study, we showed that the CART peptide binds specifically to PC12 rat pheochromocytoma cells in both the native and differentiated into neuronal phenotype. Two biologically active forms, CART(55-102) and CART(61-102), with equal biological activity, contain three disulfide bridges. To clarify the importance of each of these disulfide bridges in maintaining the biological activity of CART(61-102), an Ala scan at particular S-S bridges forming cysteines was performed, and analogs with only one or two disulfide bridges were synthesized. Inthis study, a stabilized CART(61-102) analog with norleucine instead of methionine at position 67 was also prepared and was found to bind to PC12 cells with an anorexigenic potency similar to that of CART(61-102). The binding study revea

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/GAP303%2F10%2F1368" target="_blank" >GAP303/10/1368: Nové farmakologické intervence ovlivňující energetickou rovnováhu a vývoj inzulínové rezistence/diabetu mellitu 2. typu</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Peptides

ISSN

0196-9781

e-ISSN

—

Svazek periodika

39

Číslo periodika v rámci svazku

January

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

138-144

Kód UT WoS článku

000315839300021

EID výsledku v databázi Scopus

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