Mitochondrial targeting overcomes ABCA1-dependent resistance of lung carcinoma to alpha-tocopheryl succinate
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00392315" target="_blank" >RIV/61388963:_____/13:00392315 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/86652036:_____/13:00392315 RIV/00027162:_____/13:#0000989
Výsledek na webu
<a href="http://dx.doi.org/10.1007/s10495-012-0795-1" target="_blank" >http://dx.doi.org/10.1007/s10495-012-0795-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10495-012-0795-1" target="_blank" >10.1007/s10495-012-0795-1</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Mitochondrial targeting overcomes ABCA1-dependent resistance of lung carcinoma to alpha-tocopheryl succinate
Popis výsledku v původním jazyce
alpha-Tocopheryl succinate (alpha-TOS) is a promising anti-cancer agent due to its selectivity for cancer cells. It is important to understand whether long-term exposure of tumour cells to the agent will render them resistant to the treatment. Exposure of the non-small cell lung carcinoma H1299 cells to escalating doses of alpha-TOS made them resistant to the agent due to the upregulation of the ABCA1 protein, which caused its efflux. Full susceptibility of the cells to alpha-TOS was restored by knocking down the ABCA1 protein. Similar resistance including ABCA1 gene upregulation was observed in the A549 lung cancer cells exposed to alpha-TOS. The resistance of the cells to alpha-TOS was overcome by its mitochondrially targeted analogue, MitoVES, thatis taken up on the basis of the membrane potential, bypassing the enhanced expression of the ABCA1 protein. The in vitro results were replicated in mouse models of tumours derived from parental and resistant H1299 cells. We conclude that
Název v anglickém jazyce
Mitochondrial targeting overcomes ABCA1-dependent resistance of lung carcinoma to alpha-tocopheryl succinate
Popis výsledku anglicky
alpha-Tocopheryl succinate (alpha-TOS) is a promising anti-cancer agent due to its selectivity for cancer cells. It is important to understand whether long-term exposure of tumour cells to the agent will render them resistant to the treatment. Exposure of the non-small cell lung carcinoma H1299 cells to escalating doses of alpha-TOS made them resistant to the agent due to the upregulation of the ABCA1 protein, which caused its efflux. Full susceptibility of the cells to alpha-TOS was restored by knocking down the ABCA1 protein. Similar resistance including ABCA1 gene upregulation was observed in the A549 lung cancer cells exposed to alpha-TOS. The resistance of the cells to alpha-TOS was overcome by its mitochondrially targeted analogue, MitoVES, thatis taken up on the basis of the membrane potential, bypassing the enhanced expression of the ABCA1 protein. The in vitro results were replicated in mouse models of tumours derived from parental and resistant H1299 cells. We conclude that
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
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Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)
Ostatní
Rok uplatnění
2013
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Apoptosis
ISSN
1360-8185
e-ISSN
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Svazek periodika
18
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
14
Strana od-do
286-299
Kód UT WoS článku
000315086000004
EID výsledku v databázi Scopus
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