Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F13%3A00423951" target="_blank" >RIV/61388963:_____/13:00423951 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389030:_____/13:00423951

Výsledek na webu

<a href="http://dx.doi.org/10.1155/2013/535049" target="_blank" >http://dx.doi.org/10.1155/2013/535049</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1155/2013/535049" target="_blank" >10.1155/2013/535049</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

Popis výsledku v původním jazyce

The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to s

Název v anglickém jazyce

Genotoxin Induced Mutagenesis in the Model Plant Physcomitrella patens

Popis výsledku anglicky

The moss Physcomitrella patens is unique for the high frequency of homologous recombination, haploid state, and filamentous growth during early stages of the vegetative growth, which makes it an excellent model plant to study DNA damage responses. We used single cell gel electrophoresis (comet) assay to determine kinetics of response to Bleomycin induced DNA oxidative damage and single and double strand breaks in wild type and mutant lig4 Physcomitrella lines. Moreover, APT gene when inactivated by induced mutations was used as selectable marker to ascertain mutational background at nucleotide level by sequencing of the APT locus. We show that extensive repair of DSBs occurs also in the absence of the functional LIG4, whereas repair of SSBs is seriously compromised. From analysis of induced mutations we conclude that their accumulation rather than remaining lesions in DNA and blocking progression through cell cycle is incompatible with normal plant growth and development and leads to s

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/LD13006" target="_blank" >LD13006: Molekulární podstata odpovědí rostlin na UV-B záření</a><br>

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMED RESEARCH INTERNATIONAL

ISSN

2314-6133

e-ISSN

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Svazek periodika

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Číslo periodika v rámci svazku

ID 535049

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000328834200001

EID výsledku v databázi Scopus

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