Precise estimation of HPHT nanodiamond size distribution based on transmission electron microscopy image analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F14%3A00429945" target="_blank" >RIV/61388963:_____/14:00429945 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1016/j.diamond.2014.04.002" target="_blank" >http://dx.doi.org/10.1016/j.diamond.2014.04.002</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.diamond.2014.04.002" target="_blank" >10.1016/j.diamond.2014.04.002</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Precise estimation of HPHT nanodiamond size distribution based on transmission electron microscopy image analysis

Popis výsledku v původním jazyce

Fluorescent nanodiamonds (NDs) are extremely promising fluorescent probes and sensors in experimental biology. For these applications, NDs obtained from high-pressure, high-temperature (HPHT) synthesis are used. Commercial HPHT NDs are polydisperse and of irregular shape. In this work, we used image analysis of transmission electron microscopy (TEM) micrographs to determine the actual size distribution of an HPHT ND sample with high precision. We developed novel TEM sample preparation procedure, which includes hydrophilization of a carbon-coated TEM grid by UV/ozone treatment and subsequent charge reversal and charge-controlled adsorption of nanoparticles. This method results in NDs spread evenly on the grid and radically improves the quality of TEM samples. Image analysis of TEM micrographs of commercial NDs revealed an unexpectedly high abundance of sub-10 nm NDs in the sample. This method can also be used for preparing TEM samples of other negatively charged nanoparticles, such as c

Název v anglickém jazyce

Precise estimation of HPHT nanodiamond size distribution based on transmission electron microscopy image analysis

Popis výsledku anglicky

Fluorescent nanodiamonds (NDs) are extremely promising fluorescent probes and sensors in experimental biology. For these applications, NDs obtained from high-pressure, high-temperature (HPHT) synthesis are used. Commercial HPHT NDs are polydisperse and of irregular shape. In this work, we used image analysis of transmission electron microscopy (TEM) micrographs to determine the actual size distribution of an HPHT ND sample with high precision. We developed novel TEM sample preparation procedure, which includes hydrophilization of a carbon-coated TEM grid by UV/ozone treatment and subsequent charge reversal and charge-controlled adsorption of nanoparticles. This method results in NDs spread evenly on the grid and radically improves the quality of TEM samples. Image analysis of TEM micrographs of commercial NDs revealed an unexpectedly high abundance of sub-10 nm NDs in the sample. This method can also be used for preparing TEM samples of other negatively charged nanoparticles, such as c

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CC - Organická chemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Diamond and Related Materials

ISSN

0925-9635

e-ISSN

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Svazek periodika

46

Číslo periodika v rámci svazku

Jun

Stát vydavatele periodika

CH - Švýcarská konfederace

Počet stran výsledku

4

Strana od-do

21-24

Kód UT WoS článku

000337985900004

EID výsledku v databázi Scopus

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